Product Pathways - Cell Cycle / Checkpoint
Cyclin E2 Antibody #4132
|4132S||100 µl (10 western blots)||---||In Stock||---|
|4132P||40 µl (4 western blots)||---||In Stock||---|
|4132||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Chicken.
Specificity / Sensitivity
Cyclin E2 Antibody detects endogenous levels of total cyclin E2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human cyclin E2. Antibodies are purified by protein A and peptide affinity chromatography.
Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).
- Lauper, N. et al. (1998) Oncogene 17, 2637-2643.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761.
- Ewen, M.E. (2000) Genes Dev. 14, 2265-2270.
- Won, K.A. and Reed, S.I. (1996) EMBO J. 15, 4182-4193.
- Koepp, D.M. et al. (2001) Science 294, 173-177.
- Welcker, M. et al. (2003) Mol. Cell 12, 381-392.
- Ye, X. et al. (2004) J. Biol. Chem. 279, 50110-50119.
- Cai, Z. et al. (2011) Cancer Res 71, 2466-75. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.