Product Pathways - Neuroscience
GFAP (GA5) Mouse mAb #3670
|3670S||100 µl (10 western blots)||---||In Stock||---|
|3670P||40 µl (4 western blots)||---||In Stock||---|
|3670||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||50||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
GFAP (GA5) Mouse mAb detects endogenous levels of total GFAP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord.
Western blot analysis of extracts from mouse and rat brain, using GFAP (GA5) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using GFAP (GA5) Mouse mAb.
Confocal immunofluorescent analysis of SNB19 (positive, left) and HeLa (negative, right) cells, using GFAP (GA5) Mouse mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3).
- Davies, J.E. et al. (2008) J Biol 7, 24. Applications: IF-IC (In Cells).
- Lee, Y. et al. (2013) Nat Neurosci , . Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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