Product Pathways - Protein Translation
mTOR Antibody #2972
|2972S||100 µl (10 western blots)||---||In Stock||---|
|2972||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||289||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Pig, Horse.
Specificity / Sensitivity
mTOR Antibody detects endogenous levels of total mTOR. It cross-reacts weakly with some other proteins based on Western analysis.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2481 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells (starved for 16 hours), untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) antibody #2971 (upper) or mTOR Antibody (lower).
Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or mTOR siRNA (+). mTOR was detected using mTOR Antibody #2972, and eIF4B was detected using eIF4B Antibody #3592. The mTOR Antibody confirms silencing of mTOR expression, and the eIF4B Antibody is used to control for loading and siRNA specificity.
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
- Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
- Brown, E.J. et al. (1994) Nature 369, 756-8.
- Sabatini, D.M. et al. (1994) Cell 78, 35-43.
- Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
- Dennis, P.B. et al. (2001) Science 294, 1102-5.
- Fang, Y. et al. (2001) Science 294, 1942-5.
- Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
- Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
- Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.
- Suzuki, A. et al. (2004) Mol. Cell. Biol. 24, 3526-3535. Applications: Western Blotting.
- Takahara, T. et al. (2006) J Biol Chem 281, 28605-14. Applications: Western Blotting.
- Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91. Applications: Western Blotting.
- Shavlakadze, T. et al. (2010) J Cell Sci , . Applications: Western Blotting.
- Chen, C.H. et al. (2011) Sci Signal 4, ra10. Applications: Western Blotting.
- Lee, J.W. et al. (2010) PLoS One 5, e15394. Applications: Western Blotting.
- Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting.
- Gao, D. et al. (2011) Mol Cell 44, 290-303. Applications: Western Blotting.
- Izumi, N. et al. (2012) Cancer Sci 103, 50-7. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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