Product Class: Restriction Endonuclease

NruI-HF®
neb31 cloned at NEB recombinant engineered time saver dil_A 37 No CpG dam

NruI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10128085. Learn more

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB high fidelity restriction endonuclease that recognizes the sequence TCG^CGA

Product Introduction

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance
  • 100% activity in rCutSmart Buffer
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Reduced star activity
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)
  • Restriction Enzyme Cut Site: TCG/CGA
Catalog # Size Concentration
R3192S 1000.0 units 20000 units/ml
R3192L 5000.0 units 20000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What does HF® refer to following the name of a restriction enzyme?
  2. When should I choose the HF version of an enzyme?
  3. When is star activity a concern?
  4. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  5. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  6. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
  7. Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?