Product Class: Restriction Endonuclease

BmtI

 

icon_cloned icon_recomb icon_neb3_1 dil_B-grey icon_incTemp37 icon_HI_65
BmtI_cutsite

Product Introduction

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. 

Catalog # Size Concentration
R0658S 300 units 10,000 units/ml

Product Information

Description

High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

An E.coli strain that carries the cloned BmtI gene from Bacillus megaterium S2 (S.K. Degtyarev).

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
NEBuffer™ 3.1 -20 10 X
Product Categories:
restriction-endonucleases-b
Applications:
Restriction Enzyme Digestion

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of pXba in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ 3.1
Incubate at 37°C

1X NEBuffer™ 3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 μg/ml BSA
(pH 7.9 @ 25°C)

Activity in NEBuffers

CutSmart® Buffer: 100%
NEBuffer 1.1: 100%
NEBuffer 2.1: 100%
NEBuffer 3.1: 100%

Diluent Compatibility

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 min

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Notes

  1. BmtI is an neoschizomer of NheI.

Faqs & Tech Tips

FAQs

  1. What effect does BSA have on the performance of NEB’s restriction enzymes when included in the new buffers?
  2. How can I access the old NEBuffer Activity Chart?
  3. Why is my Restriction Enzyme not cutting DNA?
  4. Why do I see a DNA smear on an agarose gel after a restriction digest?
  5. Why do I see additional DNA bands on my gel after a restriction digest?
  6. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

Protocols & Manuals

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Double Digest Protocol with Standard Restriction Enzymes

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.