Product Class: Restriction Endonuclease

AfeI
neb31 cloned at NEB recombinant dil_B 37 65 Heat CpG

AfeI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10180738. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.


NEB restriction endonuclease that recognizes the sequence AGC_^GCT
Isoschizomers | Single Letter Code

Product Introduction

Catalog # Size Concentration
R0652S 200.0 units 10000 units/ml
R0652L 1000.0 units 10000 units/ml

Featured Videos

View Video Library
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    NEB® TV Ep. 15 – Applications of Restriction Enzymes

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    Restriction Enzyme Digest Problem: Too Many DNA Bands

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    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is AfeI activity sensitive to dam, dcm or mammalian CpG methylation?
  2. How many base pairs should be added at the end of a PCR primer next to the AfeI recognition site to guarantee that AfeI will cut properly?
  3. Does AfeI have any neoschizomers?
  4. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  5. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  6. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
  7. Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?

Troubleshooting

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