Product Class: Restriction Endonuclease

MscI
neb31 cloned at NEB recombinant dil_C 37 80 Heat dcm

MscI has been reformulated, and now also includes Recombinant Albumin (rAlbumin), beginning with Lot #10215288. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence TGG_^CCA

Product Introduction

  • 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Restriction Enzyme Cut Site: TGG/CCA
Catalog # Size Concentration
R0534S 250.0 units 5000 units/ml
R0534L 1250.0 units 5000 units/ml
R0534M 1250.0 units 25000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
  3. Restriction Digest Protocol
  4. Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
  5. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is MscI affected by methylation?
  2. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?