DrdI
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0530S |
DrdI - 5.000 units/ml |
300 units ( 5000 units/ml ) | - | Unavailable in your region | |
R0530L |
DrdI - 5,000 units/ml |
1.500 units ( 5000 units/ml ) | - | Unavailable in your region |
DrdI

Catalog # | Size | Concentration |
---|---|---|
R0530S | 300 units | 5,000 units/ml |
R0530L | 1,500 units | 5,000 units/ml |
Description
Product Source
Deinococcus radiodurans (R. Morgan)Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) | Concentration | |
CutSmart® Buffer | -20 | 10X |
Properties and Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X CutSmart® Buffer
Incubate at 37°C
1X CutSmart® Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9 @ 25°C
Usage Concentration
5,000 units/ml
Activity in NEBuffers
NEBuffer 1.1: 25%NEBuffer 2.1: 50%
NEBuffer 3.1: 10%
CutSmart® Buffer: 100%
Diluent Compatibility
Storage Temperature
-20°CStorage Conditions
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping
Heat Inactivated
YesQuality Control
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.- Exonuclease Activity (Radioactivity Release):
The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides. - Ligation and Recutting (Terminal Integrity):
After an over-digestion of DNA with a restriction endonuclease the percentage of the DNA fragments ligated with T4 DNA ligase and the percentage that can be recut are determined by agarose gel electrophoresis. - Non-Specific DNase Activity (16 hour):
The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Supporting Documents
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- R0530S_L_v1_0181211
- R0530S_L_v1_0181303
- R0530S_L_v1_0181309
- R0530S_L_v1_0181402
- R0530S_L_v1_0181408
- R0530S_L_v2_0191501
- R0530S_L_v2_0191507
- R0530S_L_v2_0191512
- R0530S_L_v2_0201605
- R0530S_L_v2_0241703
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
DrdI
CutSmart® Buffer
Notes
- Star activity may result from a glycerol concentration of >5%
- Do degenerate recognition sites need to be palindromic?
- What effect does BSA have on the performance of NEB’s restriction enzymes when included in the new buffers?
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- How can I access the old NEBuffer™ Activity Chart?
- I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
- Why is my Restriction Enzyme not cutting DNA?
- Why do I see a DNA smear on an agarose gel after a restriction digest?
- Why do I see additional DNA bands on my gel after a restriction digest?
- How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
Companion Products
Materials Sold Separately
The supporting documents available for this product can be downloaded below.
MSDS
CutSmart® Buffer
MSDS
DrdI