Product Class: Restriction Endonuclease

TaqI-v2
neb31 cloned at NEB recombinant engineered time saver dil_B 65 No dam

TaqI-v2 has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10211223. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence T^CG_A

Product Introduction

  • Time-Saver™ qualified for digestion in 5-15 minutes
  • 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Restriction Enzyme Cut Site: T/CGA
Catalog # Size Concentration
R0149S 4000.0 units 20000 units/ml
R0149T 4000.0 units 100000 units/ml
R0149L 20000.0 units 20000 units/ml
R0149M 20000.0 units 100000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Why isn't TaqI-v2 cutting?
  2. What is the molecular weight of TaqI-v2?
  3. Does a certain pH range inhibit TaqI-v2  activity?
  4. Does TaqI-v2 recognize mutliple sites?
  5. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  6. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting