Product Class: Restriction Endonuclease

PstI
neb31 cloned at NEB recombinant time saver dil_C 37 80 Heat

PstI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10237495. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence C_TGCA^G

Product Introduction

Catalog # Size Concentration
R0140S 10000.0 units 20000 units/ml
R0140T 10000.0 units 100000 units/ml
R0140L 50000.0 units 20000 units/ml
R0140M 50000.0 units 100000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the activity of PstI at 25°C?
  2. Is PstI used in special techniques?
  3. When is star activity a problem for PstI?
  4. What is the molecular weight of PstI?
  5. Is PstI blocked by methylation?
  6. Is PstI inhibited by dUTP incorporated at the site?
  7. Does spermidine increase activity?
  8. Are there specific surrounding sequences that produce slow or resistant sites?
  9. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  10. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  11. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

Tech Tips

Blocked by some types of methylation often found in plant DNA.