Product Class: Restriction Endonuclease

AluI

 

icon_cloned icon_recomb icon_timesaver5 icon_CutSmart dil_B-grey icon_incTemp37 heat inactivation
Alu-I-cutsite_1

Product Introduction

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

Catalog # Size Concentration
R0137L 5,000 units 10,000 units/ml
R0137S 1,000 units 10,000 units/ml

Product Information

Description


Product Source

An E. coli strain that carries the cloned AluI gene from Arthrobacter luteus (ATCC 21606).

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
CutSmart® Buffer -20 10 X
Product Categories:
Restriction Endonucleases: A,
Time-Saver™ Qualified Restriction Enzymes
Applications:
Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
Restriction Enzyme Digestion

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X CutSmart® Buffer
Incubate at 37°C

1X CutSmart® Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
(pH 7.9 @ 25°C)

Activity in NEBuffers

CutSmart® Buffer: 100%
NEBuffer 1.1: 25%
NEBuffer 2.1: 100%
NEBuffer 3.1: 50%

Diluent Compatibility

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 min

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Faqs & Tech Tips

FAQs

  1. What is the activity of AluI at 25°C?
  2. Does AluI cleave ssDNA?
  3. Are there any common problems with AluI?
  4. What is the molecular weight of AluI?
  5. Does a certain salt or salt level inhibit activity of AluI?
  6. What effect does BSA have on the performance of NEB’s restriction enzymes when included in the new buffers?
  7. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  8. How can I access the old NEBuffer Activity Chart?
  9. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  10. What effect does BSA have on the performance of NEB's restriction enzymes when included in the new buffers?
  11. My restriction enzyme used to work well in the old NEBuffer but the new Performance chart indicates it has lower activity even though the only difference is the addition of BSA and removal of DTT to the new buffers. Why?
  12. Has NEB used any enzymes in Chromatin Conformation Capture techniques, such as 3C or HiC? 
  13. Why is my Restriction Enzyme not cutting DNA?
  14. Has NEB used any enzymes in Chromatin Conformation Capture techniques, such as 3C or HiC? 
  15. Why do I see a DNA smear on an agarose gel after a restriction digest?
  16. Why do I see additional DNA bands on my gel after a restriction digest?
  17. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
  18. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?

Tech Tips

Restriction Enzymes in Chromatin Conformation Capture
PradhanSriharsa

Protocols & Manuals

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Double Digest Protocol with Standard Restriction Enzymes
  3. Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
  4. Protocol for Digestion Prior to droplet digital PCR (ddPCR)

Other Tools & Resources

Selection Charts

Usage Guildelines & Tips

Troubleshooting Guides

Restriction Enzyme Troubleshooting Guide

InteractiveTools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.