Product Class: Other

PCR Marker

Product Introduction

  • Size range: 50 bp to 766 bp
  • Value pricing
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes

 

Catalog # Size Concentration
N3234S 100.0 gel lanes (0.1 ml) 300 µg/ml
N3234L 500.0 gel lanes (0.5 ml) 300 µg/ml

Protocols, Manuals & Usage

Protocols

  1. End-labeling Protocol
  2. Suggested Loading Protocol for DNA Ladders & Markers

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Can I use Midori Green with the DNA Ladders from NEB?
  4. Why are there extra bands visible on polyacrylamide gels?
  5. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  6. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Other Products You May Be Interested In