ShortCut® RNase IIIProduct information
ShortCut RNase III converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA).
- Isolated from a recombinant source
- Useful for gene silencing and target validation
- Supplied with 10X Reaction Buffer
ShortCut® RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells (1–3). 1.5 units (1 µl) of ShortCut RNase III is sufficient to convert 1 µg of dsRNA into siRNA suitable for RNA interference in mammalian cells.
Product SourceAn E. coli strain containing a genetic fusion of the E. coli RNase III gene (rnc) and the gene coding for maltose binding protein (MBP).
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Shortcut Reaction Buffer||10 X|
|10X EDTA||-20||500 mM|
|Glycogen RNase-Free||10 mg/ml|
- RNA Modification
Advantages and Features
- Gene silencing
- Target validation
Properties and Usage
Unit DefinitionOne unit is the amount of enzyme required to digest 1 µg of dsRNA to siRNA in 20 minutes at 37°C in a total reaction volume of 50 µl.
1X ShortCut Reaction Buffer
Incubate at 37°C
Usage Concentration1 - X
500 mM NaCl
10 mM Tris-HCl
0.5 mM EDTA
1 mM DTT
pH 8.0 @ 25°C
Unit Assay ConditionsOne unit is the amount of enzyme required to digest 1 μg of dsRNA to siRNA in 20 minutes at 37°C in a total reaction volume of 50 μl.
- Morlighem, J.E. et al. (2007). Biotechniques. 42, 599-606.
- Yang, D. et al. (2002). Proc. Natl. Acad. Sci. USA. 99, 9942-9947.
- Calegari, F. et al. (202). Proc. Natl. Acad. Sci. USA. 99, 14236-14240.
- Donze, O. and Picard, D. (2002). Nucleic Acids Res. 30, e46.
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Quality & Safety
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
ShortCut® RNase III
Shortcut Reaction Buffer
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Research Use Only
- This product is sold for research use only and not for resale in any form. Commercial use of this product may require a license. For license information, please contact the Licensing Office, New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938.
The supporting documents available for this product can be downloaded below.