Product Class: Kit

NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

The small size of this product was discontinued on December 15, 2021. If you would like to purchase a small size, an alternative is NEB #E7645S.

Product Introduction

This original version of the Ultra DNA Library Prep Kit enables library preparation from 5 ng – 1 µg of input DNA in a streamlined workflow. However, we now recommend use of the more recent NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645), which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 µg).
Catalog # Size Concentration
E7370S 24.0 reactions
E7370L 96.0 reactions

Protocols, Manuals & Usage

Protocols

  1. Protocol for use with NEBNext Ultra DNA Library Prep Kit for Illumina (E7370)
  2. Where can I find protocols for using NEBNext® Ultra DNA Library Prep Kit and Modules?
  3. Protocol for use with NEBNext Ultra DNA Library Prep Kit for Illumina (E7370)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. Can AMPure XP beads used for clean-up steps be left in the PCR reactions?
  2. For what type of sample prep/library construction can I use the NEBNext Ultra DNA Library Prep kit for Illumina for?
  3. What type and how much starting material do I need to use when preparing libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina?
  4. Is size selection required and what is the recommended method?
  5. Can I use the NEBNext Ultra DNA Library Prep Kit for Illumina reagents for preparing libraries for sequencing on the Roche 454 GS FLX & SOLiD 5500?
  6. Can I use the NEBNext Ultra DNA Library Prep Kit for Illumina reagents for preparing libraries for sequencing on the ABI SOLiD 4 instrument or on the Ion Torrent PGM?
  7. Can NEB provide any of the reagents for the cluster generation step or the actual sequencing run?
  8. How do I know the concentration of my index primers? 
  9. Which NEBNext Oligos can be used with this library prep kit?
  10. Can I use this NEBNext kit with adaptors and primers from other vendors than NEB?
  11. Are these reagents available in a customized format, larger format or 96 well formats?

Troubleshooting

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