Product Class: Other

NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

Having trouble opening your tubes? NEB Tube Opener can help!

Product Introduction

NEB 5-alpha F'Iq Competent E. coli is a derivative of the popular DH5α.

  • Extremely high transformation efficiency suitable for toxic gene cloning
  • Tight expression control (lacIq)
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Catalog # Size Concentration
C2992H 20.0 x 0.05 ml
C2992I 6.0 x 0.2 ml

Protocols, Manuals & Usage

Protocols

  1. 5 Minute Transformation Protocol (C2992)
  2. High Efficiency Transformation Protocol (C2992)

Usage & Guidelines

Application Notes

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Which competent cell strains are compatible with Gateway® Cloning?
  2. Does plasmid size affect transformation efficiency (C2992)?
  3. How long should I incubate cells on ice after DNA has been added (NEB #C2992H and NEB #C2992I)?
  4. How should I calculate the transformation efficiency (C2992)?
  5. What are the solutions/recipes (C2992)?
  6. What are the strain properties (C2992)?
  7. What is the difference between NEB #C2992H and NEB #C2992I?
  8. What is the optimal heat shock time for this strain (NEB #C2992H and NEB #C2992I)?
  9. Which strain of Competent E.coli should I use for general cloning?
  10. Can I store competent cells at -20°C instead of -80°C?
  11. Which kind of transformation tubes should be used?
  12. What volume of DNA can be added into competent cells?
  13. What is the shelf life for this strain (NEB #C2992H and NEB #C2992I)?
  14. Are NEB's competent cells compatible with the “Mix & Go" protocol?
  15. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
  16. How should fragments be prepared for assembly using NEBuilder HiFi?

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