NEB® 5-alpha Competent E. coli (High Efficiency)
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
C2987I |
NEB 5-alpha Competent E. coli (High Efficiency) |
6 x 0.2 ml | - | Unavailable in your region | |
C2987H |
NEB 5-alpha Competent E. coli (High Efficiency) |
20 x 0,05 ml | - | Unavailable in your region | |
C2987P |
NEB 5-alpha Competent E. coli (High Efficiency) |
96 wells | - | Unavailable in your region | |
C2987U |
NEB 5-alpha Competent E. coli (High Efficiency) |
96 X 50 UL | - | Unavailable in your region | |
C2987R |
NEB 5-alpha Competent E. coli (High Efficiency) |
384 wells | - | Unavailable in your region |
NEB® 5-alpha Competent E. coli (High Efficiency)
Also available in Electrocompetent format.
Catalog # | Size | Concentration |
---|---|---|
C2987P | 1 x 96 well plate (20 µl/well) | |
C2987R | 1 x 384 well plate (10 µl/well) | |
C2987I | 6 x 0.2 ml/tube | |
C2987H | 20 x 0.05 ml/tube | |
C2987U | 96 x 50 μl/tube (12 x 8-tube strips) |
Description
Highlights
- DH5α™ derivative
- Free of animal products
- Transformation efficiency:
For C2987H, C2987I, C2987P and C2987U: 1 - 3 x 109 cfu/μg pUC19 DNA
For C2987R: 1 - 5 x 108 cfu/μg pUC19 DNA - Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA2)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene
- Reduced recombination of cloned DNA (recA1)
- K12 Strain
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For C2987R, Outgrowth Medium 1.5 is provided.
Genotype
fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) | Concentration | |
pUC19 Vector | -20 | 0.05 ng/μl |
SOC Outgrowth Medium | 4 | 1X |
Advantages and Features
Applications





Properties and Usage
Antibiotics for Plasmid Selection | Working Concentration |
---|---|
Ampicillin | 100 μg/ml |
Carbenicillin | 100 μg/ml |
Chloramphenicol | 33 μg/ml |
Kanamycin | 30 μg/ml |
Streptomycin | 25 μg/ml |
Tetracycline | 15 μg/ml |
Storage Temperature
-80°CShipping Notes
- Ships on dry ice
Quality Control
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.- Transformation Efficiency:
The competent cells are tested for transformation efficiency and pass minimum release criteria. Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 μg of plasmid into a given volume of competent cells.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Supporting Documents
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- C2987H_I_v1_4491604
- C2987H_I_v1_4741606
- C2987H_I_v1_4511606
- C2987H_I_v1_4501607
- C2987H_I_v1_4621607
- C2987H_I_v1_4631607
- C2987H_I_v1_3751506
- C2987H_I_v1_3921508
- C2987H_I_v1_3991509
- C2987H_I_v1_4141510
- C2987H_I_v1_4171511
- C2987H_I_v1_4601607
- C2987U_v1_4641608
- C2987H_I_v1_4661608
- C2987P_v1_4581608
- C2987H_I_v1_4601608
- C2987H_I_v1_4601609
- C2987H_I_v1_4681609
- C2987U_v1_4641609
- C2987H_I_v1_4181512
- C2987H_I_v1_4391603
- C2987H_I_v1_4471604
- C2987H_I_v1_4671609
- C2987H_I_v1_4691609
- C2987H_I_v1_4701609
- C2987H_I_v1_4711609
- C2987H_I_v1_4731609
- C2987P_v1_4581609
- C2987H_I_v1_4741610
- C2987H_I_v1_4791610
- C2987H_I_v1_4821610
- C2987P_v1_4881611
- C2987U_v1_4901611
- C2987H_I_v1_4751610
- C2987H_I_v1_4751611
- C2987H_I_v1_4781612
- C2987H_I_v1_4921611
- C2987H_I_v1_4801611
- C2987H_I_v1_5041612
- C2987U_v1_4901612
- C2987H_I_v1_4951607
- C2987H_I_v1_4591607
- C2987H_I_v1_4831612
- C2987H_I_v1_4851612
- C2987P_v1_4881612
- C2987H_I_v1_4911612
- C2987H_I_v1_4941702
- C2987H_I_v1_4991702
- C2987H_I_v1_5011702
- C2987P_v1_4881703
- C2987H_I_v1_5101701
- C2987H_I_v1_5191702
- C2987U_v1_5121703
- C2987H_I_v1_5021703
- C2987H_I_v1_5051703
- C2987H_I_v1_4951703
- C2987H_I_v1_5091704
- C2987H_I_v1_5131704
- C2987H_I_v1_5201704
- C2987H_I_v1_5071704
- C2987H_I_v1_5151704
- C2987H_I_v1_5341704
- C2987U_v2_5121705
- C2987H_I_v1_5201705
- C2987H_I_v1_5161705
- C2987H_I_v1_5211706
- C2987H_I_v1_5321706
- C2987P_v2_5111706
- C2987U_v2_5201706
- C2987H_I_v1_3331411
- C2987H_I_v1_5221706
- C2987H_I_v1_5261706
- C2987H_I_v1_5311706
- C2987U_v2_5371706
- C2987H_I_v1_5081706
- C2987H_I_v1_5081707
- C2987H_I_v1_5271707
- C2987H_I_v1_5181707
- C2987U_v2_5371708
- C2987H_I_v1_5231707
- C2987H_I_v1_5281707
- C2987H_I_v1_5461707
- C2987H_I_v1_5481708
- C2987P_v2_5111708
- C2987H_I_v1_5241708
- C2987H_I_v1_5181708
- C2987H_I_v1_5251708
- C2987H_I_v1_5291708
- C2987U_v2_5501708
- C2987H_I_v1_5411708
- C2987H_I_v1_5471708
- C2987H_I_v1_5411709
- C2987H_I_v1_5451709
- C2987H_I_v1_5351709
- C2987H_I_v1_5421709
- C2987H_I_v1_5501710
- C2987H_I_v1_5511709
- C2987H_I_v1_5521709
- C2987P_v2_5111710
- C2987H_I_v1_5421710
- C2987H_I_v1_5541709
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
NEB® 5-alpha Competent E. coli (High Efficiency)
pUC19 Vector
SOC Outgrowth Medium
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
- C2987Datasheet-Lot205
- C2987Datasheet-Lot206
- C2987Datasheet-Lot207
- C2987Datasheet-Lot209
- C2987Datasheet-Lot210
- C2987Datasheet-Lot215
- C2987Datasheet-Lot219
- C2987Datasheet-Lot221
- C2987Datasheet-Lot222
- C2987Datasheet-Lot211
- C2987Datasheet-Lot213
- C2987Datasheet-Lot216
- C2987Datasheet-Lot217
- C2987Datasheet-Lot230
- C2987Datasheet-Lot231
- C2987Datasheet-Lot232
- C2987Datasheet-Lot239
- C2987Datasheet-Lot240
- C2987Datasheet-Lot241
- C2987Datasheet-Lot242
- C2987Datasheet-Lot243
- C2987Datasheet-Lot244
- C2987Datasheet-Lot251
- C2987Datasheet-Lot252
- C2987Datasheet-Lot254
- C2987Datasheet-Lot256
- C2987Datasheet-Lot257
- C2987Datasheet-Lot262
- C2987Datasheet-Lot268
- C2987Datasheet-Lot269
- C2987Datasheet-Lot270
- C2987Datasheet-Lot271
- C2987Datasheet-Lot272
- C2987Datasheet-Lot274
- C2987Datasheet-Lot276
- C2987Datasheet-Lot278
- C2987Datasheet-Lot291
- C2987Datasheet-Lot292
- C2987Datasheet-Lot2831402
- C2987Datasheet-Lot285
- C2987Datasheet-Lot3001404
- C2987Datasheet-Lot3031405
- C2987Datasheet-Lot3051405
- C2987Datasheet-Lot3181405
- C2987Datasheet-Lot3081405
- C2987Datasheet-Lot3171405
- C2987Datasheet-Lot3071405
- C2987Datasheet-Lot3071407
- C2987Datasheet-Lot3091406
- C2987Datasheet-Lot3101406
- C2987Datasheet-Lot3191406
- C2987Datasheet-Lot3191407
- C2987Datasheet-Lot3111408
- C2987Datasheet-Lot3131408
- C2987Datasheet-Lot3161408
- C2987Datasheet-Lot3271408
- C2987HDatasheet-Lot3271409
- C2987IDatasheet-Lot3261408
- C2987Datasheet-Lot3261410
- C2987Datasheet-Lot3381409
- C2987HDatasheet-Lot3281410
- C2987HDatasheet-Lot3281410
- C2987Datasheet-Lot3301410
- C2987PDatasheet-Lot3311410
- C2987HDatasheet-Lot3311410
- C2987HDatasheet-Lot3311410
- C2987IDatasheet-Lot3351410
- C2987HDatasheet-Lot3331411
- C2987HDatasheet-Lot3491412
- C2987IDatasheet-Lot3401412
- C2987Datasheet-Lot3441501
- C2987IDatasheet-Lot3441411
- C2987HDatasheet-Lot3341501
- C2987HDatasheet-Lot3521412
- C2987HDatasheet-Lot3541501
- C2987IDatasheet-Lot3471501
- C2987HDatasheet-Lot3461502
- C2987HDatasheet-Lot3481502
- C2987HDatasheet-Lot3631502
- C2987HDatasheet-Lot3651502
- C2987IDatasheet-Lot3531502
- C2987HDatasheet-Lot3601502
- C2987HDatasheet-Lot3831505
- C2987IDatasheet-Lot3731504
Notes
- For C2987H, C2987I, C2987P and C2987U, SOC Outgrowth Medium is provided.
For C2987R, Outgrowth Medium 1.5 is provided. - CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
- STORAGE AND HANDLING:
Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
SOC medium can be stored at either 4°C or Room Temperature depending on how fast it will be used. Storing at Room Temperature is convenient and adequate for short term usage (weeks to a couple of months). For long term storage, we recommend storing at 4°C. Please note that Outgrowth Medium 1.5 supplied with C2987R (1 x 384 well plate format) must only be stored at Room Temperature or crystals will form.
- Can I store competent cells at -20°C instead of -80°C?
- Do you carry competent cells which are compatible with Gateway® Cloning?
- How can I increase transformation efficiency?
- How long should I incubate cells on ice after DNA has been added (NEB #C2987H and NEB #C2987I)?
- How should I calculate the transformation efficiency (C2987)?
- What are the solutions/recipes (C2987)?
- What are the strain properties (C2987)?
- What is the difference between NEB #C2987H and NEB #C2987I?
- What is the difference between NEB #C2988J and NEB #C2987H?
- What is the optimal heat shock time for this strain (NEB #C2987H and NEB #C2987I)?
- What type of competent cells are suitable for transformation of DNA constructs created using Gibson Assembly?
- Which strain of Competent E. coli should I use for general cloning?
- Which kind of transformation tubes should be used?
- What volume of DNA can be added into competent cells?
- Are NEB's competent cells compatible with the “Mix & Go" protocol?
- What is the shelf life for this strain (NEB #C2987H and NEB #C2987I)?
- Can the 96-well plate format of NEB 5-alpha Competent E.coli, NEB #C2987P, be separated into smaller sections?
- How does the transformation efficiency of the 96-well plate format (NEB #C2987P) compare to the other formats?
- What is the optimal heat shock time for the 96-well plate format NEB 5-alpha Competent E.coli (NEB #C2987P)?
- I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
- What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder® HiFi DNA Assembly Master Mix?
- How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
- 5 Minute Transformation Protocol (C2987H/C2987I)
- High Efficiency Transformation Protocol (C2987H/C2987I)
- High Efficiency Transformation Protocol for 96-well format (C2987P)
- 14 Minute Transformation Protocol (C2987H/C2987I)
- High Efficiency Transformation Protocol for 96-tube format (C2987U)
- High Efficiency Transformation Protocol for 384-well format (C2987R)
Application Notes
Companion Products
- NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
- NEB® 5-alpha Electrocompetent E. coli
- NEB® 5-alpha F' Iq Competent E. coli (High Efficiency)
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
-
The supporting documents available for this product can be downloaded below.
MSDS SOC Outgrowth MediumMSDS pUC19 Vector