Product Class: Reaction Buffer

OneTaq® Standard Reaction Buffer Pack

Catalog #SizeConcentration
B9022S8 (4 x 2.0ml)5X


5X OneTaq Standard Reaction Buffer is optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer has been formulated for robust yields with minimal optimization. The OneTaq Standard Reaction Buffer is recommended for most routine and/or AT-rich amplicons (i.e. Lambda, etc.) or complex amplicons with up to ~65% GC content.

Properties and Usage

Storage Temperature


1X Buffer Components

1X Buffer Components

20mM Tris-HCl
22mM NH4Cl
22mM KCl
1.8mM MgCl2
0.06% IGEPAL® CA-630
0.05% Tween® 20
pH 8.9@25°C

Quality Control

Quality Assurance Statement

  • The reaction buffer is free of detectable nucleases.

Supporting Documents

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.
  1. The buffer arrived thawed. Are the buffer and enzyme still active?