Product Class: Other

NEBuffer™ 3

 

Product Introduction

New England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity.
 
This NEBuffer minus BSA formulation is available for those enzymes requiring NEBuffers without BSA.
 
For the BSA-containing formulation (which is included with NEB restriction enzymes), select NEBuffer 3.1 (NEB #B7203).
Catalog # Size Concentration
B7003S 5 ml 10 X

Product Information

Description

New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1 (NEB #B7201), NEBuffer 3.1 (NEB #B7203) and CutSmart Buffer (NEB #B7204) are available.
Product Categories:
Restriction Endonuclease Buffers & Diluents,
Buffer Products
Applications:
Restriction Enzyme Digestion

Properties and Usage

Storage Temperature

-20°C

1X Master Mix Composition

100 mM NaClpH 7.9 @ -20°C50 mM Tris-HClpH 7.9 @ -20°C10 mM MgCl2pH 7.9 @ -20°C1 mM DTTpH 7.9 @ -20°C

1X Master Mix Composition

100mM NaCl (pH 0 @ 0°C)pH 0 @0°C50mM Tris-HCl (pH 0 @ 0°C)pH 0 @0°C10mM MgCl2 (pH 0 @ 0°C)pH 0 @0°C1mM DTT (pH 0 @ 0°C)pH 0 @0°C

Faqs & Tech Tips

FAQs

  1. Why do I see additional DNA bands on my gel after a restriction digest?
  2. Why is my Restriction Enzyme not cutting DNA?
  3. Why do I see a DNA smear on an agarose gel after a restriction digest?
  4. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

Protocols & Manuals

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Interactive Tools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.