NucleoSpin Gel & PCR Clean-upProduct information
• Two applications one kit, one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL → highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)
|10 preps||-||Unavailable in your region|
|50 Columns||-||Unavailable in your region|
|50 preps||-||Unavailable in your region|
|250 Columns||-||Unavailable in your region|
|240 preps||-||Unavailable in your region|
|250 preps||-||Unavailable in your region|
The NucleoSpin® Gel and PCR Clean-up procedure is the easiest way to purify DNA fragments from agarose gels as well as for direct purification of PCR products. The kit includes one buffer for both applications, which contains a pH indicator displaying you the correct pH for optimal kit performance. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin® Gel and PCR Clean-up Columns provide convenient performance for PCR clean-up: After addition of Binding Buffer NTI the mixture is applied onto the silica membrane. Contaminations are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction the agarose gel slice is dissolved in high-salt Buffer NT and applied to a NucleoSpin® Gel and PCR Clean-up Column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).
NucleoSpin Gel and PCR Clean-up procedure
Binding Buffer NTI with pH indicator
The optimal pH to bind even small DNA fragments to the silica membrane is about 5–6. Binding Buffer NTI is sufficiently buffered to maintain this pH. However, to be sure that the pH is right even for samples with extreme alkaline pH or high buffer concentration, a pH indicator has been added. The pH indicator does not interfere with DNA binding and is completely removed during the purification. The yellow color is also beneficial for gel extractions,making it easy to identify undissolved pieces of agarose.
For restoring the correct conditions add more Buffer NTI or 4 M sodium acetate (NaAc, pH 5.0), or small amounts of hydrochloric acid (HCl) until the color switches back to yellow.
Colors of the indicator
|Format||Mini spin columns|
|Sample material||<400 µl PCR reaction mixture
<400 mg TAE/TBE agarose gel
|Fragment size||50 bp-20 kbp|
|Elution volume||15-50 µl|
|Preparation time||10 min/6 preps|
|Binding capacity||25 µg|
The supporting documents available for this product can be downloaded below.