Where can I find protocols for using the NEBNext® Enzymatic Methyl-seq Kit? (NEB #E7120, E7125, E7140)


Protocols for using NEBNext® Enzymatic Methyl-seq Reagents can be found in the product's manual.


Click here for all protocols for use with the NEBNext® Enzymatic Methyl-seq Kit NEB #E7120:

https://www.neb.com/en-us/products/e7120-nebnext-enzymatic-methyl-seq-kit#Protocols,%20Manuals%20&%20Usage_Manuals

The NEBNext® Enzymatic Methyl-seq Kit manual contains the following protocols:

Section 1 Protocol for use with Standard Size Libraries (370–420 bp)

Section 2 Protocol for use with Large Size Libraries (470–520 bp)

Section 3 Index Guidelines


Click here for the protocol for use with the NEBNext® Enzymatic Methyl-seq Conversion Module NEB #E7125:

https://www.neb.com/en-us/products/e7125-nebnext-enzymatic-methyl-seq-conversion-module#Protocols,%20Manuals%20&%20Usage_Manuals

The NEBNext® Enzymatic Methyl-seq Kit manual contains the following protocol:

Protocol for the EM-seq Conversion Module


Click here for all protocols for use with the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) NEB #E7140:

https://www.neb.com/en-us/products/e7140-nebnext-multiplex-oligos-for-enzymatic-methyl-seq-unique-dual-index-primer-pairs#Protocols,%20Manuals%20&%20Usage_Manuals

The NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) manual contains the following protocols:

Section 1 Setting up PCR Reactions

Section 2 A: Index Pooling Guidelines, 24 Reaction Kit

B: Index Pooling Guidelines, 96 Reaction Kit