PhosphoPlus® MEK1/2 (Ser217/221) Antibody KitProduct information
|1 Kit (10 western blots)||-||Unavailable in your region|
Product Pathways - MAPK Signaling
PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit #9120
|9120S||1 Kit (10 western blots)||---||In Stock||---|
|9120||carrier free and custom formulation / quantity||email request|
|Kit Includes||Quantity||Applications||Reactivity||Homology†||Mol. Wt.||Isotype|
|Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154||100 µl||W, IP||H, M, R, Mk, Dm||C||45 kDa||Rabbit|
|MEK1/2 (D1A5) Rabbit mAb #8727||100 µl||W, IF-IC, F||H, M, R, Mk, Dm||Hm, X, Z, B, Dg, Pg, Ce||45 kDa||Rabbit IgG|
|MEK1/2 HeLa Control Cell Extracts #9160||200 µl||W|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||W||Goat|
|Anti-biotin, HRP-linked Antibody #7075||100 µl||W||All||Goat|
|20X LumiGLO® Reagent and 20X Peroxide #7003||5 ml each||W|
|Biotinylated Protein Ladder Detection Pack #7727||100 µl|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Dm=D. melanogaster, All=All Species Expected
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (upper), or MEK1/2 Antibody #9122 (lower).
Western blot analysis of extracts from various cell lines using MEK1/2 (D1A5) Rabbit mAb.
Western blot analysis of extracts from Hela cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) RmAb #9154 (upper) or MEK1/2 (D1A5) Rabbit mAb #8727 (lower).
Flow cytometric analysis of Jurkat cells using MEK1/2 (D1A5) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Confocal immunofluorescent analysis of HeLa cells using MEK1/2 (D1A5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of MEK1/2 at Ser217/221.
Specificity / Sensitivity
Phospho-MEK1/2 (Ser217/221) Antibody detects endogenous levels of MEK1/2 only when phosphorylated at serines 217 and 221. It will also react with MEK1/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221. MEK1/2 (D1A5) Rabbit mAb recognizes endogenous levels of total MEK1 and MEK2 proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 and synthetic peptide corresponding to residues surrounding Ala220 of human MEK1 protein.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Xiao, D. and Singh, S.V. (2002) Cancer Res. 62, 3615-3619. Applications: Western Blotting.
- Watanabe, H. et al. (2001) J. Biol. Chem. 276, 14466-14473. Applications: Western Blotting.
- Jost, M. et al. (2001) J. Biol. Chem. 276, 6320-6326. Applications: Western Blotting.
- DeWindt, L. J. et al. (2000) . Journal of Biological Chemistry 275, 13571-13579. Applications: Western Blotting.
- Soga, S. et al. (1998) . Journal of Biological Chemistry 273, 822-828. Applications: Western Blotting.
- Galbiati, F. et al. (1998) . European Molecular Biology Organization (EMBO) 17, 6633-6648. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.