Phospho-c-Kit (Tyr719) AntibodyProduct information
Product Pathways - Tyrosine Kinase / Adaptors
Phospho-c-Kit (Tyr719) Antibody #3391
|3391L||300 µl (30 western blots)||---||In Stock||---|
|3391S||100 µl (10 western blots)||---||In Stock||---|
|3391T||20 µl (2 western blots)||---||In Stock||---|
|3391||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse||Endogenous||120, 145||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-c-Kit (Tyr719) Antibody detects endogenous levels of c-Kit only when phosphorylated at tyrosine 719. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr719 of mouse c-Kit. Antibodies are purified by protein A and peptide affinity chromatography.
c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
- Martin, F.H. et al. (1990) Cell 63, 203-11.
- Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
- Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
- Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
- Nocka, K. et al. (1990) EMBO J 9, 1805-13.
- Hirota, S. et al. (1998) Science 279, 577-80.
- Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
- Warshamana-Greene, G. S. et al. (2004) Mol Cancer Ther 3, 527-536. Applications: Western Blotting.
- Bosbach, B. et al. (2012) Proc Natl Acad Sci U S A 109, E2276-83. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 3073 Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 3308 c-Kit (Ab81) Mouse mAb
- 3310 c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 3074 c-Kit (D13A2) XP® Rabbit mAb
- 3392 c-Kit Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.