Protocols

2736:

Western Blotting,  Immunoprecipitation (Magnetic)

Background

Fes/Fps and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases (1,2). Fes and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains (3). Fes/Fps was originally identified as an oncogene from avian (Fps) and feline (Fes) retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. Mutations may activate the Fps kinase and thereby contribute to cancer (4). However, recent data strongly suggests that the c-Fes protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer (5).

1. Smithgall, T.E. et al. (1998) Crit Rev Oncog 9, 43-62.
2. Greer, P. (2002) Nat Rev Mol Cell Biol 3, 278-89.
3. Sangrar, W. et al. (2005) Cancer Res 65, 3518-22.
4. Ley, T.J. et al. (2003) Proc Natl Acad Sci U S A 100, 14275-80.
5. Delfino, F.J. et al. (2006) J Biol Chem 281, 8829-35.

Application References

• Alvarez-Errico, D. et al. (2011) J Immunol 187, 5577-86. Applications: Western Blotting.

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