Detection of human B7-H3/CD276 by western blot.Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-B7-H3/CD276 antibody A304-367A (lot A304-367A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. "
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