OneTaq® DNA Polymerase from NEB

Robust amplification across a wide range of templates

OneTaq® and OneTaq® Hot Start DNA polymerases provide an optimized blend of Taq and Deep VentR® DNA polymerases. Both are available as stand-alone enzyme or in a master mix format.

Benefits

• Increased fidelity - 2X higher than Taq alone
• Robust amplification
• Convenience - Minimal optimization, regardless of a template’s GC content

Phusion® High-Fidelity DNA Polymerase from NEB

The well-known high-fidelity polymerase

Phusion® High-Fidelity DNA Polymerase is trusted for many years and offers robust performance for a wide range of templates. The fidelity is >50 times higher than Taq, what means very low error rates.

Benefits

• Fidelity – High fidelity (> 50X higher than Taq)
• Versatility – Can be used for routine PCR as well as long or difficult templates
• Amplicon length – Robust amplifications up to 20 kb for simple templates

Nucleotides from NEB

Quality deoxynucleotide and ribonucleotide solution sets and mixes are also available from NEB.

Q5™ High-Fidelity DNA Polymerase from NEB

The new standard for robust performance plus high fidelity

Q5™ High-Fidelity DNA Polymerase sets a new standard for both fidelity and performance. With the highest-fidelity amplification available (>100 times higher than Taq), Q5™ DNA Polymerase results in ultra-low error rates. The one single Q5™ reaction buffer is designed to provide superior performance with minimal optimization across a broad range of amplicons, regardless of GC content. Q5™ is the most robust and quickest high-fidelity polymerase on the market.

Benefits

• Fidelity – Highest fidelity (>100X higher than Taq)
• Coverage – Superior performance for a broad range of amplicons (from high AT to high GC)
• Speed – Shortest extension times: 10 s/kb for simple templates and 10-30 s/kb for complex templates
• Amplicon length – Robust amplifications up to 20 kb for simple templates
• Convenience – Normal and Hot Start enzyme available as stand-alone or in master mix format

To find the perfect polymerase for your PCR reaction, check out the Polymerase Selection Chart.

FrameStar® PCR plates from 4titude

Optimum PCR results plus easy and reliable handling

FrameStar® PCR plates combine the advantages of thin-walled polypropylene tubes for optimum PCR with a rigid frame portion for more reliable handling. In contrast to standard plastics, the rigid two-component frame technology maximizes thermal stability of plates at high temperatures. The reduced thermal expansion minimizes sample loss due to evaporation from corner positions and outer rows of wells during PCR, allowing you to downscale reagent volumes to increase efficiency and to lower your reagent costs. FrameStar® PCR plates are available for a wide range of PCR cyclers, qPCR cyclers and sequencers.

FrameStar® Break-A-Way from 4titude

Tube strips or plates – the choice is yours

FrameStar® Break-A-Way is a 96-well PCR plate that can easily be divided into smaller strips of 8 tubes, ensuring no tubes are wasted.

4LAB™ Automated Low Volume Liquid Handling from 4titude

Affordable, easy-to-use, and precision

The 4LAB™ is an automated, high-precision pipetting system specifically designed for low-volume PCR/qPCR reaction set-up. It is available as a 4-position and a 6-position workstation. For good PCR results, small volumes of reagents need to be dispensed accurately and consistently - however manual reaction setup is tedious, time consuming and prone to human error, and most robotic systems are very expensive and complex to program. The intuitive set-up and programming of the 4LAB™ is designed for researchers without prior robotic experience and will save your laboratory time and money from day 1.

Benefits

• Money saving – Affordable and it reduces reagent costs by using low volume PCR plates
• Easy and Convenient Use – Intuitive software comes with built-in protocols that can be quickly and easily modified.
• Easy Service – Pipetting modules are simple to remove.
• Flexible – Available as 4-position and a 6-position workstation.
• Accuracy and Precision – Excellent results for qPCR standard curves and replicates.

4s3™ semi-automatic Thermal Sealer from 4titude

Eliminate sample loss and reduce reaction volumes

The 4s3™ is a semi-automatic thermal sealer compatible with a wide range of seals and plate designs and heights. Temperature and time settings are easily optimized to produce a 100% seal, this minimizes evaporation and eliminate sample loss. Results are more reliable and uniform, and you can save on save on reagent costs as reaction volumes can be reduced to as little as 10 μl.

Benefits

• Flexible - Compatible with all PCR plate formats
• Save time - Always a 100% seal
• Save Money - Eliminates sample loss and reduces your reaction volume

Labcycler from SensoQuest

The most innovative thermocyclers The SensoQuest team has developed and produced thermocyclers since the 1990s. The Labcycler range represents the latest generation of cyclers, with many new and innovative features. The labcycler is available in different sizes and with different thermoblocks like the Triple Block 3x21 and the thermoblocks 48, 96 and 384.

Benefits

• Highest accuracy - 6 separately controlled peltier elements with zone temperature regulation, continuously self-calibrating temperature measuring circuitry, and automated lid
• Intuitive interface - Large TFT Touch Screen: quick and easy protocol programming
• Flexibility - Flexible block changing system
• Reliable - 5 years warranty

NucleoSpin® Gel & PCR Clean-up Kit from MACHEREY-NAGEL

The easiest way to purify DNA from gels or PCR reactions

For optimal restriction or ligation, DNA needs to be cleaned up after the PCR reaction. The two-in-one NucleoSpin® Gel & PCR Clean-up kit is the easiest way to purify DNA fragments either directly from PCR reactions or after separation on agarose gels. There is a convenient single buffer for both reactions, and since the elution volume is only 15 μl there is generally no need to concentrate the DNA further.

Benefits

• Convenience - One buffer for both PCR clean-up and gel extraction
• Time Saving - Elution volume only 15ul, no extra steps needed to concentrate

NucleoSpin® Gel and PCR Clean-up covers all polymerase buffer systems

A PCR fragment with a size of 165 bp was amplified using different DNAPolymerase reaction mix formulations (a–c). Additional primers were added and the mixture was purified using competitor kits from Q, S, and R. The elution was performed with 25 μL. For analysis the complete eluate was loaded onto a 1% TAE agarose gel (u: unpurified). In comparison to MACHEREY-NAGEL NucleoSpin® Gel and PCR Clean-up (MN) all other kits show lower recovery or inefficient removal of primers. With MN only 1 kit is needed instead of a separate kit for the gel and a kit for the PCR.

HF™ Restriction Enzymes from NEB

Experience a new level of flexibility in reaction conditions

More than 200 Restriction Enzymes from New England Biolabs are 100% active in the single CutSmart Buffer, which includes BSA for added simplicity. The engineered line of High Fidelity (HF™) restriction enzymes have the same specificity as their established counterpart with the benefit of reduced star activity and rapid digestions.

Benefits

• One-buffer Convenience – Ideal for double digests
• High Performance – Significantly reduced star activity, even after O/N incubation
• High Speed – Quick set-up and fast digestions (5-15 min)

EcoRI-HF™ shows significantly reduced star activity, as compared to enzyme from other manufacturer that digest DNA in 5 minutes.

EcoRI-HF™ shows no star activity when used in 5 minutes or overnight. 50 μl reactions were set up using 1 μg of Lambda DNA, 1 μl of enzyme and recommended reaction buffer. Digests were incubated at 37°C. Marker M is the 1kb DNA Ladder (NEB# N3232).

Extensive selection of performanceoptimized DNA ligases

With over 37 years of experience in the development and production of enzymes for molecular biology, NEB offers 10 different DNA ligase products – more than any other supplier. This extensive selection of high-quality, and performanceoptimized DNA ligases and ligase master mixes will streamline your cloning experiments.

Benefits

• Save time - Fast ligations
• Ensure successful ligations - Robust ligation efficiency
• Maximize your transformation efficiencies - High specificity ligation
• Experience extreme purity – High and consistent level of purity
   

Competent Cells from NEB

Find the perfect cells for your needs

For cloning experiments choose from several high efficiency competent cell strains. For example the NEB Turbo Competent E. coli is a chemically competent E. coli cell line suitable for high efficiency transformation and rapid colony growth.

Benefits

• Fast - Colonies visible after 6.5 hours
• Reliable - Free of animal products
• Efficient - 1-3 x 109 cfu/μg pUC19 DNA

High-yield, large scale DNA purification

NucleoBond^® Xtra is a new generation of anion exchangers for increased yield and purity. Based on the patented NucleoBond® technology, NucleoBond® Xtra typically yields ≥ 250 μg (Midi) or ≥ 1000 μg (Maxi) of ultra-pure plasmid DNA.

Benefits

• Save your money - This technology results in 100% more yield, use a midi prep instead of a maxi prep.
• Save your time - Achieve 2x CsCl purified transfection-grade plasmid DNA in less than 30 minutes.

Plasmid DNA was isolated following each manufacturer’s protocol using the maximum culture volume with high plasmid content. Yield of plasmid DNA was determined after precipitation (NucleoBond® Xtra) and after elution from the column (competitor kits) respectively.

Pre-mixed and ready to load

Quick-Load® 100 bp and 1kb DNA Ladders are pre-mixed, readyto- load molecular weight markers containing bromophenol blue as a tracking dye.