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Generating a clonal GFP cell line typically requires first sorting GFP positive cells with a cell sorter. Then GFP positive cells are cultured for several days to recover from the high pressure damage caused by the cell sorter. Clonal GFP cells are finally obtained by manual limited dilution at 1 cell per well. In this application note, Namocell Single Cell Dispenser was used to directly isolate single GFP positive cells into 96-well cell culture plate. Due to the low pressure of the Namocell Single Cell Dispenser, clonal GFP cell lines can be generated in a single step.