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Abundant RNAs: Can you really have too much RNA?

RNA sequencing (RNA-seq) faces a unique challenge. In total RNA, the majority of the RNA species present will be ribosomal RNAs (rRNAs) or sample-specific messenger RNAs (mRNAs). Since the abundant RNAs obscure the true biological phenomena that are being studied, they should be removed prior to sequencing, thus reducing the number of reads required to obtain the information of interest.

 

NEBNext® RNA Depletion Workflow


NEBs NEBNext RNA Depletion products rely on an RNase H-based workflow to specifically and efficiently degrade and remove the abundant RNAs from the sample, preparing it for library prep. In addition to the original kit for rRNA depletion from human, mouse, and rat samples, NEB now offers two new RNA depletion kits:

 

Request your free sample and test yourself

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