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The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.
E7300 NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)
E7330 NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)
E7580 NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)
Watch as Product Developers Daniela Munafo, Ph.D. and Brett Robb, Ph.D. recount NEB's breakthrough method for RNA Sample Preparation for Next Gen Sequencing.
NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA and target enrichment of DNA. Available for a wide range of sample types, NEBNext products are provided in user-friendly formats including kits and modules, with bulk or customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.
Each set of reagents is functionally validated together through construction of a library from a DNA or RNA reference sample that is then sequenced on the appropriate platform. Whenever a new lot of any of the reagents is introduced into a kit, the entire kit is re-validated by library construction and sequencing.
NEBNext reagents are available for sample preparation for Illumina®, Ion Torrent™ and 454™ sequencing platforms.
NEBNext® is a registered trademark of New England Biolabs, Inc.
Illumina® is a registered trademark of Illumina, Inc.
Ion Torrent™is a trademark of Life Technologies, Inc.
454™ is a trademark of Roche.