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Tools for researching the SARS-CoV-2 virus

 

The COVID-19 public health crisis affects everyone in our global community, and many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this virus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against COVID-19 by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support.

During this COVID-19 pandemic you can expect the same quality and support from us as before. We have implemented multiple measures to keep our employees and business partners safe, and at the same time to maintain the continuity of supply of our products and support to our customers. We are working closely with our suppliers to identify and mitigate risks in the supply of products. Furthermore we are monitoring the situation and following the guidance of national and international health organizations and governmental authorities around the world.

On this page you will find a selection of our products that can be used for virus research. However we have more products that can be relevant, please let us know if there is anything we can do to help your research.

Are you in SARS-CoV-2 research? Let us know how we can support your research and receive a discounted quotation.

 

Request your discount

 



Viral purification products

MACHEREY-NAGEL (MN)

Multiple labs have included MACHEREY-NAGEL's NucleoSpin and NucleoMag kits for isolation of viral RNA into their workflows, among them the two WHO reference laboratories, the Institut Pasteur Paris (France) and the National Institute of Health (Thailand). We are supporting all labs by providing guidelines for sample preparation and nucleic acid extraction with MN kits in compliance with WHO and CDC recommendations. The recommendations include all our NucleoSpin and NucleoMag kits suitable for extraction of viral RNA. MN protocol recommendations for respiratory samples (swabs, sputum, bronchoalveolar lavage). Please note, BIOKÉ distributes MACHEREY-NAGEL in the Netherlands only (click here for other countries)

Products:

  • NucleoSpin® RNA Virus* (740956) > see comments below
  • NucleoSpin® Dx Virus* (CE-IVD) (740895) > see comments below
  • NucleoSpin® Virus (740983)
  • NucleoMag® Pathogen (744210)
  • NucleoMag® Virus (744800)
  • NucleoSpin® 8 / 96 Virus (740451)

*For the following reasons MN recommends to use NucleoSpin® RNA Virus instead of NucleoSpin® Dx Virus for SARS-CoV-2 detection:

  1. The CE-IVD mark only applies for plasma/serum, does not include respiratory samples!
  2. NucleoSpin® Dx Virus kit contains Proteinase K and 1.5 mL Tubes, which are not required for SARS-CoV-2 isolation.

The quality assurance management from MN provided a comparison sheet stating the equivalence of NucleoSpin Dx Virus and NucleoSpin RNA Virus kit components.

 

Downloads

 

Viral RNA isolation from water samples

Monitoring coronavirus in sewage could help to estimate the total number of infections. Infectious diseases that are excreted in urine or faeces, such as SARS-CoV-2, can be tracked by analyzing wastewater. Worldwide many researchers started to use wastewater testing to be able to early on identify a potential reoccurrence of the virus in certain communities. Routine wastewater surveillance might be an efficient, non-invasive tool to detect new COVID-19 infections and might help to limit the health and economic damaged caused by COVID-19. To support our customers with this promising approach, MN R&D scientists came up with recommendations for extracting RNA from water samples using MACHEREY-NAGEL kits NucleoBond® RNA Soil Midi kit, NucleoSpin® RNA Stool, NucleoMag® DNA/RNA Water.


New England Biolabs (NEB)

NEB has a new protocol for Monarch® RNA Purification: RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit. This protocol for the Monarch Total RNA Miniprep Kit has been validated with buccal swabs and saliva samples. We have also confirmed the compatibility of this workflow with viral transport medium using reference samples that do not require cell and/or viral envelope lysis. Request a sample

  • Quickly and easily purify up to 100 µg of high quality total RNA (>20 nt)
  • Purified RNA is suitable for downstream reactions, such as RT-qPCR and library prep for next gen sequencing
  • Elute in as little as 30 µl (RNA samples can be further concentrated following purification using our Monarch RNA Cleanup columns or kits)

 


Other RNA products

MACHEREY-NAGEL

  • NucleoProtect RNA stabilization reagent (740400) for cells and tissues
  • One phase total RNA extraction from all kinds of sample with NucleoZOL (740404)

Please note, BIOKÉ distributes MACHEREY-NAGEL in the Netherlands only

 


Rapid Detection of Coronavirus (COVID-19) RNA

New England Biolabs

The WarmStart® Colorimetric LAMP 2X Master Mix (DNA &RNA) (NEB #M1800) is a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Fast, clear pink-to-yellow visible detection of amplification

  • WarmStart® feature inhibits activity at room temperature

  • Learn how Colorimetric LAMP was used for rapid detection of coronavirus (COVID-19) RNA in our recent publication

 

Facilitating Detection of SARS-CoV-2: Precursor Studies with Luna® RT-qPCR and Colorimetric RT-LAMP Reagents

This new application note is presented in the context of the COVID-19 global pandemic caused by the SARS-CoV-2 coronavirus. To help accelerate diagnostics development efforts, we demonstrate the detection of synthetic SARS-CoV-2 viral RNA targets using NEB’s RT-qPCR and colorimetric RT-LAMP reagents. The colorimetric RT-LAMP work is a follow-up on a recent publication by researchers at NEB, in collaboration with researchers at the Wuhan Institute of Virology in China, demonstrating the potential of colorimetric RT-LAMP detection of SARS-CoV-2 viral RNA purified from patient samples.

Download this application note

 


Homogenization of cells and tissues

Syntec International

The Medimachine II can be used for the homogenization of cells and tissues to extract biomolecules for molecular biology or protein biochemistry applications. Medimachine II homogenizator provides high quality results for extraction of molecules for the analysis of chemical residues, virus, bacterias etc.

 


Measuring Viral Cytopathic Effects (CPE) in real time

Agilent Technologies

The accurate measurement of viral activity plays an essential role for viral vaccine development and infectious disease studies. When infected with a virus, host cells often display changes that are collectively referred to as a cytopathic effect (CPE) which include cell shrinkage or enlargement, deterioration/lysis, cell fusion, and the formation of inclusion bodies. The rapid, sensitive, and high throughput xCELLigence Real-Time Cell Analysis Assay is ideal for infectious viral assays. Learn more

  • Get Automatic and Rapid Results
    Monitor viral CPE without using agar, dye, fixative, or labels, over time scales ranging from minutes to days
  • Improve Lab Safety
    Greatly reduce workload and manual handling of samples, minimizing risk of exposure to infectious material
  • Highly Sensitive
    Obtain quantitative kinetics for the entire virus life cycle with exquisite sensitivity and reproducibility
  • Objective
    Eliminate the subjectivity of data analysis due to visual interpretation
  • Scalable and Adaptable
    Compatible with both 96 and 384 well formats as well as disease relevant and primary cells

 


qPCR reagents

New England Biolabs

The Luna Universal One-Step RT-qPCR kits offer exceptional sensitivity, reproducibility and RT-qPCR performance. Luna® Universal Probe One-Step RT-qPCR Kit (NEB #E3006) & Luna Universal One-Step RT-qPCR Kit (NEB #E3005) are also suitable for high-throughput qPCR and RT-qPCR.

  • Rapid, sensitive and precise probe or dye-based qPCR detection and quantitation of RNA targets
  • Novel, thermostable Luna WarmStart RT increases reaction specificity
  • Carryover prevention supported: reaction mixes contain dUTP
  • Compatible with high-throughput automation workflows

 

With the Luna Cell Ready Lysis Module (NEB #E3032), you bypass RNA purification and go direct to RT-qPCR or LAMP analysis

  • Coordinated cell lysis, RNA release and genomic DNA removal are all performed in a 15-minute protocol
  • Pair with our Luna Universal One-Step RT-qPCR kits or WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) for streamlined assays

 

High-throughput qPCR and RT-qPCR Workflows: Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB Luna Reagents

Luna qPCR reagents are a great choice for automated high-throughput qPCR and RT-qPCR applications. Highlights from a new application note include:

  • Luna qPCR and One-Step RT-qPCR are fully compatible with Echo-mediated acoustic liquid handling
  • Linear, accurate quantitation, sensitive detection, exceptional reproducibility, and robust room temperature stability during automated workflows
  • Echo-mediated reaction setup enables fast, accurate, high-throughput assembly of qPCR experiments
  • An adapted Luna Cell Ready protocol allows direct lysis of cells in Echo-qualified cell culture source plates for automated, high-throughput workflow

Read this application note

 


qPCR plates

4titude (part of Brooks)

qPCR accuracy matters now more than ever. Our partner 4titude provides high-quality qPCR plates for a wide range of cyclers.

Below you will find a selection of 2-component plates that are available for the Roche Lightcycler:
FrameStar® 384 Well Skirted PCR Plate, Roche Style (4ti-0381)
FrameStar® 384 Well Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0382)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style (4ti-0951)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0952)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity (4ti-0954)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity, Plus qPCR Seal (4ti-0954/0560)

The qPCR seals (4ti-0560) are available in the combi packs and separately.

More plates for the Lightcycler and for other cyclers can be found via the Brooks Plate Wizard



Sequencing products

New England Biolabs

Products for Oxford Nanopore Technologies® Sequencing: NEB offers multiple products, including NEBNext sample prep modules, that are being recommended in a number of third party COVID-19 sequencing protocols. These protocols include “PCR tiling of COVID-19 virus” in Oxford Nanopore Technologies’ Nanopore Community, and the ARTIC protocol.

Products for Illumina® Sequencing: The following reagents are available for sequencing on the Illumina platform:

  • NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770)

 


Antibody products for researching the host-immune response to SARS-CoV-2

Cell Signaling Technology (CST)

CST has well-validated research antibodies and small molecule compounds, which can help researchers unveil the pathogenesis of viral disease and advance research and development of assays and therapeutic drugs against COVID-19.

Browse all validated CST tools for researching the host-immune reponse to viruses like SARS-CoV-2

To enable the scientific community to accelerate critical research in the search for a cure, we are offering qualifying scientists working on SARS-CoV-2 up to 50% off on selected products from Cell Signaling Technology. Learn more

 

Bethyl Laboratories

The following reagents from Bethyl Laboratories are especially relevant to COVID-19 (SARS-CoV-2) seroconversion assay development: Human IgM Antibody (goat) (A80-100A), Human IgM Antibody (rabbit) (A80-101A), Human IgG-Fc Fragment Antibody (goat) (A80-104A), Human IgG-Fc Fragment Antibody (rabbit) (A80-105A), Human IgA Antibody (goat) (A80-102A), and Human IgA Antibody (rabbit) (A80-103A). Check out Bethyl's Bulk antibody program and Bethyl can also partner with you to develop recombinant rabbit monoclonals against novel targets.

 


 

Technical Support

Please let us know if there is anything we can do to help your research

E. techsupport@bioke.com

T. +31 (0)71 7200 220 (option 4)

 

 

How can we support you?

BIOKÉ distributes these products in the Netherlands, Belgium and Luxembourg, and MACHEREY-NAGEL products in the Netherlands only

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