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The BIOKÉ webshop is closed until January 2nd, 2019. We wish you a successful 2019!
The COVID-19 public health crisis affects everyone in our global community, and many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this virus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against COVID-19 by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support.
During this COVID-19 pandemic you can expect the same quality and support from us as before. We have implemented multiple measures to keep our employees and business partners safe, and at the same time to maintain the continuity of supply of our products and support to our customers. We are working closely with our suppliers to identify and mitigate risks in the supply of products. Furthermore we are monitoring the situation and following the guidance of national and international health organizations and governmental authorities around the world.
On this page you will find a selection of our products that can be used for virus research. However we have more products that can be relevant, please let us know if there is anything we can do to help your research.
Are you in SARS-CoV-2 research? Let us know how we can support your research and receive a discounted quotation.
Multiple labs have included MACHEREY-NAGEL's NucleoSpin and NucleoMag kits for isolation of viral RNA into their workflows, among them the two WHO reference laboratories, the Institut Pasteur Paris (France) and the National Institute of Health (Thailand). We are supporting all labs by providing guidelines for sample preparation and nucleic acid extraction with MN kits in compliance with WHO and CDC recommendations. The recommendations include all our NucleoSpin and NucleoMag kits suitable for extraction of viral RNA. MN protocol recommendations for respiratory samples (swabs, sputum, bronchoalveolar lavage). Please note, BIOKÉ distributes MACHEREY-NAGEL in the Netherlands only (click here for other countries)
*For the following reasons MN recommends to use NucleoSpin® RNA Virus instead of NucleoSpin® Dx Virus for SARS-CoV-2 detection:
The quality assurance management from MN provided a comparison sheet stating the equivalence of NucleoSpin Dx Virus and NucleoSpin RNA Virus kit components.
Viral RNA isolation from water samples
Monitoring coronavirus in sewage could help to estimate the total number of infections. Infectious diseases that are excreted in urine or faeces, such as SARS-CoV-2, can be tracked by analyzing wastewater. Worldwide many researchers started to use wastewater testing to be able to early on identify a potential reoccurrence of the virus in certain communities. Routine wastewater surveillance might be an efficient, non-invasive tool to detect new COVID-19 infections and might help to limit the health and economic damaged caused by COVID-19. To support our customers with this promising approach, MN R&D scientists came up with recommendations for extracting RNA from water samples using MACHEREY-NAGEL kits NucleoBond® RNA Soil Midi kit, NucleoSpin® RNA Stool, NucleoMag® DNA/RNA Water.
NEB has a new protocol for Monarch® RNA Purification: RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit. This protocol for the Monarch Total RNA Miniprep Kit has been validated with buccal swabs and saliva samples. We have also confirmed the compatibility of this workflow with viral transport medium using reference samples that do not require cell and/or viral envelope lysis. Request a sample
Please note, BIOKÉ distributes MACHEREY-NAGEL in the Netherlands only
The WarmStart® Colorimetric LAMP 2X Master Mix (DNA &RNA) (NEB #M1800) is a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
Fast, clear pink-to-yellow visible detection of amplification
WarmStart® feature inhibits activity at room temperature
Facilitating Detection of SARS-CoV-2: Precursor Studies with Luna® RT-qPCR and Colorimetric RT-LAMP Reagents
This new application note is presented in the context of the COVID-19 global pandemic caused by the SARS-CoV-2 coronavirus. To help accelerate diagnostics development efforts, we demonstrate the detection of synthetic SARS-CoV-2 viral RNA targets using NEB’s RT-qPCR and colorimetric RT-LAMP reagents. The colorimetric RT-LAMP work is a follow-up on a recent publication by researchers at NEB, in collaboration with researchers at the Wuhan Institute of Virology in China, demonstrating the potential of colorimetric RT-LAMP detection of SARS-CoV-2 viral RNA purified from patient samples.
The Medimachine II can be used for the homogenization of cells and tissues to extract biomolecules for molecular biology or protein biochemistry applications. Medimachine II homogenizator provides high quality results for extraction of molecules for the analysis of chemical residues, virus, bacterias etc.
The accurate measurement of viral activity plays an essential role for viral vaccine development and infectious disease studies. When infected with a virus, host cells often display changes that are collectively referred to as a cytopathic effect (CPE) which include cell shrinkage or enlargement, deterioration/lysis, cell fusion, and the formation of inclusion bodies. The rapid, sensitive, and high throughput xCELLigence Real-Time Cell Analysis Assay is ideal for infectious viral assays. Learn more
The Luna Universal One-Step RT-qPCR kits offer exceptional sensitivity, reproducibility and RT-qPCR performance. Luna® Universal Probe One-Step RT-qPCR Kit (NEB #E3006) & Luna Universal One-Step RT-qPCR Kit (NEB #E3005) are also suitable for high-throughput qPCR and RT-qPCR.
With the Luna Cell Ready Lysis Module (NEB #E3032), you bypass RNA purification and go direct to RT-qPCR or LAMP analysis
Luna qPCR reagents are a great choice for automated high-throughput qPCR and RT-qPCR applications. Highlights from a new application note include:
qPCR accuracy matters now more than ever. Our partner 4titude provides high-quality qPCR plates for a wide range of cyclers.
Below you will find a selection of 2-component plates that are available for the Roche Lightcycler:
FrameStar® 384 Well Skirted PCR Plate, Roche Style (4ti-0381)
FrameStar® 384 Well Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0382)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style (4ti-0951)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0952)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity (4ti-0954)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity, Plus qPCR Seal (4ti-0954/0560)
The qPCR seals (4ti-0560) are available in the combi packs and separately.
More plates for the Lightcycler and for other cyclers can be found via the Brooks Plate Wizard
Products for Oxford Nanopore Technologies® Sequencing: NEB offers multiple products, including NEBNext sample prep modules, that are being recommended in a number of third party COVID-19 sequencing protocols. These protocols include “PCR tiling of COVID-19 virus” in Oxford Nanopore Technologies’ Nanopore Community, and the ARTIC protocol.
Products for Illumina® Sequencing: The following reagents are available for sequencing on the Illumina platform:
CST has well-validated research antibodies and small molecule compounds, which can help researchers unveil the pathogenesis of viral disease and advance research and development of assays and therapeutic drugs against COVID-19.
To enable the scientific community to accelerate critical research in the search for a cure, we are offering qualifying scientists working on SARS-CoV-2 up to 50% off on selected products from Cell Signaling Technology. Learn more
The following reagents from Bethyl Laboratories are especially relevant to COVID-19 (SARS-CoV-2) seroconversion assay development: Human IgM Antibody (goat) (A80-100A), Human IgM Antibody (rabbit) (A80-101A), Human IgG-Fc Fragment Antibody (goat) (A80-104A), Human IgG-Fc Fragment Antibody (rabbit) (A80-105A), Human IgA Antibody (goat) (A80-102A), and Human IgA Antibody (rabbit) (A80-103A). Check out Bethyl's Bulk antibody program and Bethyl can also partner with you to develop recombinant rabbit monoclonals against novel targets.
Please let us know if there is anything we can do to help your research
T. +31 (0)71 7200 220 (option 4)