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Q5® High-Fidelity DNA Polymerase
cloned at NEB recombinant hifi pcr 3

Fidelity at its finest – for over 10 years

This is product is available in a glycerol-free format. Contact us for more information.

Product Introduction

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. 

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Also available: Q5 Hot Start standalone polymerase (NEB #M0493) and master mix formats (NEB #M0492, M0494)
Catalog # Size Concentration
M0491S 100.0 units 2000 units/ml
M0491L 500.0 units 2000 units/ml

Featured Videos

View Video Library
  • Important_Tips_Q5_TN

    Important Tips for Q5® Polymerase

  • Why_Choose_Q5_TN

    Why Choose Q5® High-fidelity Polymerase?

  • BehindthePaper_SourcesofErrorinPCR_17_282X210

    Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing

  • TmImportance_VideoThumb

    Why is Tm Important in Primer Design?

  • LargeAmplicons_VideoThumb

    Tips for Amplifying Large Amplicons

  • Amplify_GC_rich_DNA_TN

    How to Amplify GC-rich DNA

  • PCRTips_VideoThumb

    5 Tips for Setting Up Your PCR

  • PCROverview_thumb

    Overview of PCR

Protocols, Manuals & Usage

Protocols

  1. PCR Using Q5® High-Fidelity DNA Polymerase (M0491)

Usage & Guidelines

Application Notes

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What are the advantages of using Q5® High-Fidelity DNA Polymerase?
  2. What are the differences between the numerous Q5® Polymerase products available?
  3. My results are not as expected. Where can I find troubleshooting help?
  4. What ends will my PCR products have?
  5. What does exonuclease activity mean for a DNA polymerase?
  6. Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
  7. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  8. How should I determine the appropriate annealing temperature for my reaction?
  9. What should the final primer concentration be in my PCR?
  10. When and how should I use the Q5® High GC Enhancer?
  11. Do other polymerases work in Q5® Reaction Buffer?
  12. There is a precipitate in the bottom of the buffer tube. Is this normal?

Troubleshooting

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