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NucleoSpin RNA

Product information

Benefits

  • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
  • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) ncluded
  • Up to 70 μg ready-to-use RNA
  • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size

Code Name Size Quantity Price
740955.10
NucleoSpin RNA
10 preps    - Unavailable in your region
740955.50S
NucleoSpin RNA II Columns
50 Columns    - Unavailable in your region
740955.50
NucleoSpin RNA
50 preps    - Unavailable in your region
740955.250S
NucleoSpin RNA Columns
250 Columns    - Unavailable in your region
740955.240C
NucleoSpin RNA for QIAcube
240 preps    - Unavailable in your region
740955.250
NucleoSpin RNA
250 preps    - Unavailable in your region
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