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NEBuilder HiFi DNA Assembly


NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible kit enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.
 

4 Reasons to choose NEBuilder HiFi 

  1. Save time – Enjoy simple and fast seamless cloning in as little as 15 minutes.
  2. Flexibility – Use one system for both "standard-size" cloning and larger gene assembly products, up to 6 fragments.
  3. Compatible with downstream applications – DNA can be used immediately for transformation or as template for PCR or RCA.
  4. Adaptable – Adapts easily for multiple DNA manipulations, including site-directed mutagenesis. 

 
NEBuilder HiFi DNA assembly offers unique features over other DNA assembly methods, including:

  • 3´- and 5´-end mismatch removal
  • Bridging of two ds-fragments with a ssDNA oligo
  • Improved methods for site-directed mutagenesis

    

Request your free sample today!    Learn more with brochures or application notes 

 
   
Not your average DNA assembly reagent

NEBuilder HiFi DNA Assembly can be used for a variety of DNA assembly methods.


 

Application Notes  Brochures  Free Sample


  
NEBuilder HiFi products

NEBuilder HiFi DNA Assembly Cloning Kit

  • Includes NEB 5-alpha competent cells
  • NEB recommends NEB 5-alpha Competent E. coli (High Efficiency, NEB #C2987) for routine assemblies of 15 kb or less
  • For repetitive sequences, NEB recommends NEB Stable Competent E. coli (NEB #C3040)


NEBuilder HiFi DNA Assembly Master Mix

  • Does not include competent cells 


 

 

 

  

Sample request NEBuilder HiFi DNA Assembly Master Mix*
 

✓ Yes, I am interested to test the NEBuilder HiFi DNA Assembly Master Mix

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*This free sample can be requested by laboratory from the Netherlands, Belgium and Luxembourg until June 30, 2017. No cash or cash equivalent. Offer may not be applied to existing, pending or prior orders. Limit one sampe per department. Free samples cannot be requests by current users of NEBuilder HiFi DNA Assembly products. 

 

 

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