New England Biolabs Restriction Enzymes
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New England Biolabs Restriction Enzymes
Inside the Numbers
With over 40 years of offering restriction enzymes to the research community, New England Biolabs (NEB) has earned the reputation of being a leader in enzyme technologies. NEB offers scientists 276 restriction enzymes, the largest selection in the industry.
New England Biolabs
Restriction Enzymes
> 205 restriction enzymes are 100% active in the single CutSmart™ buffer, making double digest reactions significantly easier to set up. Since many DNA modifying enzymes are also 100% active in CutSmart buffer, there is often no need for purification between reactions.
Enzyme | Activity in CutSmart buffer | Required Supplements |
Alkaline Phosphatase (CIP) | +++ | |
T4 DNA polymerase | +++ | |
Shrimp Alkaline Phosphatase (rSAP) | +++ | |
T4 DNA Ligase | +++ | Requires ATP |
+++ full functional activity ++ 50–100% functional activity
+ 0–50% functional activity
Visit NEBCutSmart.com for a complete overview of the DNA modifying enzymes.
Of the 20 biggest selling restriction enzymes from NEB, 19 enzymes are supplied with the CutSmart buffer and 1 is provided with its own buffer. The convenience of this unrivaled buffer compatibility means great savings to your budget and time.
Buffers supplied with the most commonly sold restriction enzymes | |||
NEB | Thermo Scientific | Promega | Roche |
20 enzymes available with just 2 buffers | 20 enzymes available with 9 different buffers | 14 enzymes evailable with 5 different buffers | 13 enzymes available with 4 different buffers |
A table with the 20 biggest selling restriction enzymes can be found here
BamHI-HF® and SalI-HF® both exhibit 100% activity in the CutSmart buffer so each enzyme works at regular concentration to obtain 100% cleavage.
Enzyme | Activity % in NEBuffers | |||
1.1 | 2.1 | 3.1 | CutSmart | |
BamHI-HF | 25% | 100% | 100% | 100% |
SalI-HF | 100% | 25% | 10% | 100% |
There is no buffer available in which both enzymes exhibit 100% activity. As these two enzymes are not compatible, Roche recommends performing a sequential digestion to get the job done.
Enzyme | Activity % in Roche buffers | ||||
A | B | L | M | H | |
BamHI | 100% | 100% | 75-100% | 100% | 25-50% |
SalI | 0-10% | 25-50% | 0-10% | 10-25% | 100% |
Example: Reduced Star activity of NEB EcoRI NEB EcoRI-HF (#R3101) shows no star activity when used for 5 minutes or overnight. 50 μl reactions were set up using 1 μg of Lambda DNA, 1 μl of enzyme (NEB EcoRI-HF or Thermo Scientific Fermentas EcoRI) and recommended reaction buffer. Digests were incubated at 37°C. |
Example: Power and purity of time-saver qualified NEB HindIII-HF NEB’s HindIII-HF (#R3104) is powerful enough to digest 1 μg of DNA in 5 minutes, but can also be used in overnight digests with no indication of nuclease contamination. Marker (M) is the 1 kb DNA Ladder (#N3232). |
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Supplier | Price 1.000 units** | Difference* | |
NEB | € 1.832,95 | ||
Thermo Scientific Fermentas | Conventional | € 1.805,10 | -2% |
FastDigest | € 19.069,74 | 90% | |
Roche | € 4.921,57 | 63% | |
Promega | € 3.686,52 | 50% |
* Average difference to NEB list price
** Price comparison (price of 1.000 units of the most-sold 20 enzymes) is based on similar size packs published in June 2014 by the suppliers on their website. Not all suppliers were able to provide all the 20 restriction enzymes, for these enzymes we used the average price.
Biggest selling New England Biolabs restriction enzymes
Cat # | Enzyme | Buffer | |
1. | R3552S | AgeI-HF | |
2. | R0558S | AscI | |
3. | R3136S |
BamHI-HF |
|
4. | R3535S | BsaI-HF | |
5. | R0176S | DpnI | |
6. | R0543S | DpnII | |
7. | R3101S | EcoRI-HF | |
8. | R3104S | HindIII-HF | |
9. | R3589S | MfeI-HF | |
10. | R3193S | NcoI-HF |
Cat # | Enzyme | Buffer | |
11. | R0111S | NdeI | |
12. | R3131S | NheI-HF | |
13. | R3189S | NotI-HF | |
14. | R0547S | PacI | |
15. | PmeI | ||
16. | R3138S | SalI-HF | |
17. | R3133S | SpeI-HF | |
18. | R0145S | XbaI | |
19. | R0146S | XhoI | |
20. | R0180S | XmaI |
= Cutsmart Buffer = Own unique NEBBuffer
290 |
Restriction enzymes |
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270 |
Recombinant enzymes |
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205 |
Enzymes exhibiting 100% activity in a single buffer |
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190 |
Time-Saver qualified enzymes |
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26 |
High-Fidelity Enzymes engineered |
Restriction enzyme master mixes