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Tools for virus research (COVID-19)

 


In December 2019 in Wuhan China, originated an outbreak of a novel coronavirus also called severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The disease it can cause is called coronavirus disease 2019, abbreviated as COVID-19. Most patients have a fever and respiratory complaints. The World Health Organization (WHO) recently declared an international public health emergency over the global outbreak of the novel coronavirus. Governments around the globe are taking measures to slow down the spreading of the virus and prevent an overload of the healthcare system.

BIOKÉ has implemented multiple measures to keep our employees and business partners safe, and at the same time to maintain the continuity of supply of our products and support to our customers. All product lines that BIOKÉ distributes are sourced from suppliers in the US and Europe. We are working closely with our suppliers to identify and mitigate risks in the supply of products. Furthermore we are monitoring the situation and following the guidance of national and international health organizations and governmental authorities around the world.

Please let us know if there is anything we can do to help your research. You can reach us via the following email addresses and phone numbers.
Ordering: order@bioke.com / +31 (0)71 7200 220 option 1
Webshop: webmaster@bioke.com / +31 (0)71 7200 220 option 2
Technical: techsupport@bioke.com / +31 (0)71 7200 220 option 4

If you have any other questions, please do not hesitate to contact us at: marketing@bioke.com



Tools for virus research

Currently scientists are working very hard to get more knowledge about the virus and develop diagnostic tests, treatments and vaccines. WHO is gathering the latest scientific findings and knowledge on coronavirus disease (COVID-19) and compiling it in a database.

BIOKÉ offers a range of different tools that can be used in virus research, see below.

Viral purification products
Other RNA products
Rapid Detection of Coronavirus (COVID-19) RNA
Homogenization of cells and tissues
Measuring Viral Cytopathic Effects (CPE) in real time
qPCR reagents
qPCR plates
Sequencing products
Antibody products in COVID-19 research

Please contact us if you have any technical questions about our products or applications (techsupport@bioke.com / +31 (0)71 7200 220 option 4)

 


Viral purification products

MACHEREY-NAGEL (MN) - Multiple labs have included MACHEREY-NAGEL´s NucleoSpin and NucleoMag kits for isolation of viral RNA into their workflows, among them the two WHO reference laboratories, the Institut Pasteur Paris (France) and the National Institute of Health (Thailand). We are supporting all labs by providing guidelines for sample preparation and nucleic acid extraction with MN kits in compliance with WHO and CDC recommendations. The recommendations include all our NucleoSpin and NucleoMag kits suitable for extraction of viral RNA. MN protocol recommendations for respiratory samples (swabs, sputum, bronchoalveolar lavage). Please note, BIOKÉ only distributes MN in the Netherlands (click here for other countries):

NucleoSpin® RNA Virus (740956)
NucleoSpin® Dx Virus (CE-IVD)* (740895)
NucleoSpin® Virus (740983)
NucleoMag® Pathogen (744210)
NucleoMag® Virus (744800)
NucleoSpin® 8 / 96 Virus (740451)

*Use of swabs, sputum and bronchoalveolar lavage are not in the scope of the certified intended use of this CE-IVD product.

Current WHO references:

Institut Pasteur Paris (Paris, France); NucleoSpin Dx Virus
National Institute of Health (Nonthaburi, Thailand); NucleoSpin RNA Virus

 


New England Biolabs (NEB) does not currently supply a kit specifically designed for viral RNA extraction. Additionally, we have not validated this Monarch® Total RNA Miniprep Kit (NEB #T2010) for RNA extraction from nasopharyngeal swabs or aspirates. However, several customers have provided feedback that they have used this kit successfully to extract viral RNA from other biological samples.

  • Quickly and easily purify up to 100 µg of high quality total RNA (>20 nt)
  • Purified RNA is suitable for downstream reactions, such as RT-qPCR and library prep for next gen sequencing
  • Elute in as little as 30 µl (RNA samples can be further concentrated following purification using our Monarch RNA Cleanup columns or kits)
  • New protocols for the use of this kit with different biological samples are being posted as they are developed. As an example, for guidance on the use of this kit with saliva, buccal swabs and nasal swabs click here.

 

Other RNA products

MACHEREY-NAGEL:

  • NucleoProtect RNA stabilization reagent (740400) for cells and tissues
  • One phase total RNA extraction from all kinds of sample with NucleoZOL (740404)

Please note, BIOKÉ only distributes MACHEREY-NAGEL in the Netherlands

 

Rapid Detection of Coronavirus (COVID-19) RNA

New England Biolabs - WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800) is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution.The mix contains a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions.

  • Easily detect amplification with a simple pink-to-yellow color change

  • Set up reactions at room temperature, as WarmStart feature inhibits activity at 25°C

  • Learn how Colorimetric LAMP was used for rapid detection of Coronavirus (COVID-19) RNA in our recent publication

 

Homogenization of cells and tissues

Syntec International - The Medimachine II can be used for the homogenization of cells and tissues to extract biomolecules for molecular biology or protein biochemistry applications. Medimachine II homogenizator provides high quality results for extraction of molecules for the analysis of chemical residues, virus, bacterias etc.

 

Measuring Viral Cytopathic Effects (CPE) in real time

Agilent - The accurate measurement of viral activity plays an essential role for viral vaccine development and infectious disease studies. When infected with a virus, host cells often display changes that are collectively referred to as a cytopathic effect (CPE) which include cell shrinkage or enlargement, deterioration/lysis, cell fusion, and the formation of inclusion bodies. The rapid, sensitive, and high throughput xCELLigence Real-Time Cell Analysis Assay is ideal for infectious viral assays. Learn more

  • Get Automatic and Rapid Results
    Monitor viral CPE without using agar, dye, fixative, or labels, over time scales ranging from minutes to days
  • Improve Lab Safety
    Greatly reduce workload and manual handling of samples, minimizing risk of exposure to infectious material
  • Highly Sensitive
    Obtain quantitative kinetics for the entire virus life cycle with exquisite sensitivity and reproducibility
  • Objective
    Eliminate the subjectivity of data analysis due to visual interpretation
  • Scalable and Adaptable
    Compatible with both 96 and 384 well formats as well as disease relevant and primary cells

 

qPCR reagents

New England Biolabs:

The Luna Universal One-Step RT-qPCR kits offer exceptional sensitivity, reproducibility and RT-qPCR performance. Luna® Universal Probe One-Step RT-qPCR Kit (NEB #E3006) & Luna Universal One-Step RT-qPCR Kit (NEB #E3005)

  • Rapid, sensitive and precise probe or dye-based qPCR detection and quantitation of RNA targets
  • Novel, thermostable Luna WarmStart RT increases reaction specificity
  • Carryover prevention supported: reaction mixes contain dUTP
  • Compatible with high-throughput automation workflows

 

With the Luna Cell Ready Lysis Module (NEB #E3032), you bypass RNA purification and go direct to RT-qPCR or LAMP analysis

  • Coordinated cell lysis, RNA release and genomic DNA removal are all performed in a 15-minute protocol
  • Pair with our Luna Universal One-Step RT-qPCR kits or WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) for streamlined assays

 

qPCR plates

qPCR accuracy matters now more than ever. Our supplier 4titude (part of Brooks) provides high-quality qPCR plates for a wide range of cyclers.

Below you will find a selection of 2-component plates that are available for the Roche Lightcycler:
FrameStar® 384 Well Skirted PCR Plate, Roche Style (4ti-0381)
FrameStar® 384 Well Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0382)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style (4ti-0951)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0952)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity (4ti-0954)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity, Plus qPCR Seal (4ti-0954/0560)

The qPCR seals (4ti-0560) are available in the combi packs and separately.

More plates for the Lightcycler and for other cyclers can be found via the Brooks Plate Wizard


Sequencing products

New England Biolabs:

Products for Oxford Nanopore Technologies® Sequencing

The following reagents are being recommended in a number of third party COVID-19 sequencing protocols, including “PCR tiling of COVID-19 virus” in Oxford Nanopore Technologies’ Nanopore Community, and the ARTIC protocol:”

Products for Illumina® Sequencing

The following reagents are available for sequencing on the Illumina platform:

  • NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770)

 

Antibody products in COVID-19 research

Cell Signaling Technology has well-validated research antibodies and small molecule compounds, which can help researchers unveil the pathogenesis of viral disease and advance research and development of assays and therapeutic drugs. Read blog. All CST products for COVID-19

The following reagents from Bethyl Laboratories are especially relevant to COVID-19 (SARS-CoV-2) seroconversion assay development: Human IgM Antibody (A80-100A), Human IgM Antibody (A80-101A), Human IgG-Fc Fragment Antibody (A80-104A), Human IgG-Fc Fragment Antibody (A80-105A), Human IgA Antibody (A80-102A), and Human IgA Antibody (A80-103A). Check out Bethyl's Bulk antibody program and Bethyl can also partner with you to develop recombinant rabbit monoclonals against novel targets.


Please let us know if there is anything we can do to help your research (techsupport@bioke.com / +31 (0)71 7200 220 option 4)


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