Product Class: Kit

EpiMark® Methylated DNA Enrichment Kit

Product Introduction

This kit offers reagent for selectively enriching double-stranded methyl-CpG DNA from fragmented genomic DNA.
  • The methyl-CpG binding domain of human MBD2 protein fused to the Fc-tail of human IgG1 (MBD2-Fc) provides high-affinity binding and greater sensitivity
  • Easy-to-use protocol yields enriched fractions in less than 2 hours
  • Enriched methylated DNA fractions can be easily ligated to double-stranded adaptors for Next Generation Sequencing
  • Each kit contains sufficient reagents for the enrichment of methylated DNA from up to 100 μg of fragmented input DNA
Catalog # Size Concentration
E2600S 25.0 reactions

Protocols, Manuals & Usage

Protocols

  1. DNA Fragmentation (E2600)
  2. Prebind MBD2a-Fc to Protein A Magnetic Beads (NEB #E2600)
  3. Capture Methylated CpG DNA (E2600)
  4. Wash Off Unbound DNA (E2600)
  5. Elute Captured Methylated CpG DNA (E2600)
  6. Capture and Elute Step for Control DNA (E2600)
  7. Downstream Analysis (NEB #E2600)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

Troubleshooting