Product Class: Other

RNA 5' Pyrophosphohydrolase (RppH)
NEB2 cloned at NEB recombinant 37

Product Introduction

RNA 5´ Pyrophosphohydrolase (RppH) removes pyrophosphate from the 5´ end of triphosphorylated RNA to leave a 5´ monophosphate RNA.

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of endonucleases, exonucleases, RNases and phosphatases
Catalog # Size Concentration
M0356S 200.0 units 5000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Decapping eukaryotic mRNA with RppH (NEB #M0356)
  2. Cappable-seq for prokaryotic transcription start site (TSS) determination
  3. Removal of pyrophosphate from 5 triphosphorylated RNA using RppH (NEB #M0356)

FAQs & Troubleshooting

FAQs

  1. Can RppH replace Tobacco Acid Pyrophosphatase (TAP) as a decapping enzyme?

Tech Tips

We strongly recommend wearing gloves, using nuclease-free tubes and reagents, and thoroughly cleaning pipettes and bench surfaces to avoid RNase contamination.

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