Product Class: Restriction Endonuclease

Nt.CviPII
neb31 cloned at NEB recombinant dil_A 37 65 Heat CpG

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence ^CCD
Isoschizomers | Single Letter Code

Product Introduction

  • Nt.CviPII is used in the NicE-Seq protocol, which is used for high resolution open chromatin profiling. Please refer to FAQs and protocols (below) for more information.
  • This is a nicking endonuclease, which generates DNA molecules that are “nicked”, rather than cleaved
  • Nicking Enzyme Cut Site: CCD (-3/none)
Catalog # Size Concentration
R0626S 40.0 units 2000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Open Chromatin Labeling and NicE-seq library construction using Nt.CviPII
  4. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  2. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  3. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

Tech Tips

Blocked by CpG methylation Enzyme has inherent exonuclease activity

Other Products You May Be Interested In