Product Class: Restriction Endonuclease

BaeI
neb31 cloned at NEB recombinant time saver dil_A 37 65 Heat CpG

SAM now included in enzyme formulation – no longer supplied as a separate vial. New reaction temperature.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence _NNNNN^NNNNNNNNNNACNNNNGTAYCNNNNNNN_NNNNN^
Isoschizomers | Single Letter Code

Product Introduction

  • Time-Saver™ qualified for digestion in 5-15 minutes
  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Restriction Enzyme Cut Site: (10/15)ACNNNNGTAYC(12/7)
Catalog # Size Concentration
R0613S 250.0 units 5000 units/ml
R0613L 1250.0 units 5000 units/ml

Featured Videos

View Video Library
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    Reduce Star Activity with High-Fidelity Restriction Enzymes

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    TIME-SAVER™ Protocol for Restriction Enzyme Digests

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    NEB® TV Ep. 15 – Applications of Restriction Enzymes

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    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

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    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

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    Why is My Restriction Enzyme Not Cutting DNA?

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    Restriction Enzyme Digest Problem: Too Many DNA Bands

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    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Does BaeI have any special requirements?
  2. Does BaeI cleave DNA twice?
  3. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  4. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  5. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  6. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
  7. In an effort to standardize reaction conditions, the recommended incubation temperature has changed for ApaI (NEB #R0114), BaeI (NEB #R0613), BsrDI (NEB #R0574) and SmaI (NEB #R0141). Which incubation temperature should I use?

Troubleshooting

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