Product Class: Restriction Endonuclease

SapI
neb31 cloned at NEB recombinant time saver dil_B 37 65 Heat

Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments).

SapI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10161947. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.


NEB restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_
Isoschizomers | Single Letter Code

Product Introduction

Catalog # Size Concentration
R0569S 250.0 units 10000 units/ml
R0569L 1250.0 units 10000 units/ml

Featured Videos

View Video Library
  • GoldenGateAssemblyWorkflow_720

    Golden Gate Assembly Workflow

  • GoldenGateDomestication_Anim_720

    Golden Gate Assembly Domestication Tutorial

  • high complexity golden gate assembly targets

    Listen to DAD (Data-optimized Assembly Design) when constructing high-complexity Golden Gate Assembly targets

  • Golden-Gate-Assembly-Tool-Tutorial

    Golden Gate Assembly Tool Tutorial

  • ReduceStarActivity_video_thumb

    Reduce Star Activity with High-Fidelity Restriction Enzymes

  • timesaverprotocol_video_thumb

    TIME-SAVER™ Protocol for Restriction Enzyme Digests

  • NEBTVEp15VLthumb280x210

    NEB® TV Ep. 15 – Applications of Restriction Enzymes

  • cuttingclose_video_thumb

    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

  • dnasmear_video_thumb

    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

  • EnzymeNotCutting_video_thumb

    Why is My Restriction Enzyme Not Cutting DNA?

  • TooManyBands_video_thumb

    Restriction Enzyme Digest Problem: Too Many DNA Bands

  • nebcloner_dd_demo_thumb

    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes
  4. 13-Fragment Golden Gate Assembly Protocol using SapI (NEB #R0569)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Why isn't SapI cutting?
  2. What sequence does SapI recognize?
  3. Is extended digestion of SapI recommended?
  4. Is SapI used for any special techniques?
  5. What is the activity of SapI at 25°C?
  6. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  7. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  8. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  9. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  10. Does NEB offer any BSA-free and/or animal origin-free restriction enzymes for linearization of plasmids for mRNA vaccine development?
  11. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

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