Product Class: Restriction Endonuclease

EcoO109I
neb31 cloned at NEB recombinant time saver dil_A 37 65 Heat dcm

EcoO109I has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10133985. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence RG^GNC_CY
Isoschizomers | Single Letter Code

Product Introduction

  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Restriction Enzyme Cut Site: RG/GNCCY

    • Catalog # Size Concentration
    R0503S 2000.0 units 20000 units/ml

    Featured Videos

    View Video Library
    • ReduceStarActivity_video_thumb

      Reduce Star Activity with High-Fidelity Restriction Enzymes

    • timesaverprotocol_video_thumb

      TIME-SAVER™ Protocol for Restriction Enzyme Digests

    • NEBTVEp15VLthumb280x210

      NEB® TV Ep. 15 – Applications of Restriction Enzymes

    • cuttingclose_video_thumb

      Restriction Enzyme Digest Protocol: Cutting Close to DNA End

    • dnasmear_video_thumb

      Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

    • EnzymeNotCutting_video_thumb

      Why is My Restriction Enzyme Not Cutting DNA?

    • TooManyBands_video_thumb

      Restriction Enzyme Digest Problem: Too Many DNA Bands

    • nebcloner_dd_demo_thumb

      Double Digestion with NEBcloner

    Protocols, Manuals & Usage

    Protocols

    1. Optimizing Restriction Endonuclease Reactions
    2. Restriction Digest Protocol
    3. Double Digest Protocol with Standard Restriction Enzymes

    Usage & Guidelines

    Tools & Resources

    Selection Charts

    Web Tools

    FAQs & Troubleshooting

    FAQs

    1. Do degenerate recognition sites need to be palindromic?
    2. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
    3. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
    4. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
    5. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

    Troubleshooting

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