Product Class: Restriction Endonuclease

HpaI
neb31 cloned at NEB recombinant dil_A 37 No CpG

HpaI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10128074. Learn more

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GTT_^AAC
Isoschizomers | Single Letter Code

Product Introduction

Catalog # Size Concentration
R0105S 500.0 units 5000 units/ml
R0105L 2500.0 units 5000 units/ml

Featured Videos

View Video Library
  • ReduceStarActivity_video_thumb

    Reduce Star Activity with High-Fidelity Restriction Enzymes

  • StandardREProtocol_video_thumb

    Standard Protocol for Restriction Enzyme Digests

  • NEBTVEp15VLthumb280x210

    NEB® TV Ep. 15 – Applications of Restriction Enzymes

  • cuttingclose_video_thumb

    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

  • dnasmear_video_thumb

    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

  • EnzymeNotCutting_video_thumb

    Why is My Restriction Enzyme Not Cutting DNA?

  • TooManyBands_video_thumb

    Restriction Enzyme Digest Problem: Too Many DNA Bands

  • nebcloner_dd_demo_thumb

    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
  3. Restriction Digest Protocol
  4. Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
  5. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. When is star activity a problem for HpaI?
  2. What do we know about star activity with Hpal I?
  3. How do you recomend using HpaI?
  4. Does a certain salt or salt level inhibit activity of HpaI?
  5. Does spermidine increase activity of HpaI?
  6. Is HpaI inhibited by dUTP incorporated at the site?
  7. Is HpaI active at 25°C?
  8. What is the molecular weight of HpaI?
  9. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  10. What is Star Activity and how can it be avoided?
  11. How can this enzyme be inactivated?
  12. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  13. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  14. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
  15. Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?

Troubleshooting

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