Product Class: Other

50 bp DNA Ladder

This product is available in our Quick-Load Purple format - enjoy the convenience of a ready-to-load format.

Product Introduction

  • Size range: 50 bp - 1350 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Easy-to-identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes


50 bp DNA Ladder visualized by ethidium bromide staining on a 3% TBE agarose gel. Mass values are for 1 µg/lane.
Catalog # Size Concentration
N3236S 200.0 gel lanes (0.1 ml) 1000 µg/ml
N3236L 1000.0 gel lanes (0.5 ml) 1000 µg/ml

Protocols, Manuals & Usage

Protocols

  1. End-labeling Protocol
  2. Suggested Loading Protocol for DNA Ladders & Markers

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Why are there extra bands visible on polyacrylamide gels?
  2. Why is the separation of the lower bands incomplete?
  3. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  4. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  5. How can I quantify the amount of DNA in each band of a marker?
  6. Can I use Midori Green with the DNA Ladders from NEB?
  7. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  8. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
  9. Can I use GelRed® with the Low Molecular Weight and 50 bp DNA Ladders from NEB?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Other Products You May Be Interested In