Product Class: Kit

PreCR® Repair Mix

Product Introduction

Recover damaged, compromised, or degraded DNA samples

  • Repair DNA prior to its use in DNA-related technologies
  • Easy-to-use protocols
  • Does not harm DNA template
Catalog # Size Concentration
M0309S 30.0 reactions
M0309L 150.0 reactions

Protocols, Manuals & Usage

Protocols

  1. Sequential Reaction Protocol for PreCR Repair Mix
  2. Standard Reaction Protocol for PreCR Repair Mix
  3. Control Reaction Protocol for PreCR Repair Mix

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Will PreCR ligate my DNA fragments?
  2. How much DNA will PreCR repair?
  3. Will treating my DNA with the PreCR Repair Mix hurt my reaction?
  4. What is the sequence of the L1 primer mix?
  5. How is the damaged DNA that you test the PreCR Repair Mix against created? Can I buy this damaged DNA separately?
  6. Why does my control reaction not give a detectable amplicon?
  7. Can I buy any of the PreCR Repair Mix components separately?
  8. Will the PreCR Repair Mix blunt the ends of the DNA?
  9. Why don’t I see the expected band from my repaired template?
  10. Is my buffer compatible and which reaction should I use?
  11. Does the PreCr Repair Mix insert random nucleotides into the sequence that it repairs?
  12. Does the PreCR Repair Mix contain any contaminating human DNA? What are the quality controls that are used to test that?
  13. Does the PreCR Repair Mix remove covalent modifications from DNA bases, such as biotin or digoxigenin? Does it repair mismatches/ extra bases in DNA?
  14. If there are gaps and nicks remaining from a ligation reaction, will the PreCR Repair Mix repair all of these, so that the DNA will be suitable for microinjection into mice, for example?
  15. Can the PreCR Repair Mix be used for paraffin-embedded DNA?
  16. The repaired DNA will be used for an Nsp1 or Sty1 digestion followed by an adapter ligation, and PCR. Do you recommend cleanup of the PreCR Repair Mix reaction prior to this process?
  17. Can the PreCR Repair Mix repair damage in both single and double stranded DNA? Or, does it require a double stranded DNA as a template?
  18. Is the addition of dNTPs necessary for the PreCR Repair Mix to work properly?
  19. If I had a DNA template with mutation sites (ie. 8-oxoguanine or deaminated cytosines) that are directly adjacent to each other on opposite strands would treatment with PreCR™ Repair Mix cause a double strand nick/break?
  20. What gap lengths can be repaired with the PreCR Repair Mix?
  21. Does the PreCR Repair Mix work with less concentrated amounts of DNA (e.g. 500pg-1ng) than the amounts recommended?
  22. When doing bisulfite treatments of templates, the subsequent PCR reactions can pose a problem as the DNA seems to be very labile after the treatment. Can the PreCR Repair Mix improve these PCR results?
  23. Is it necessary to clean the PreCR reaction prior to carrying out quantitative PCR?
  24. When working with fragments, will the ends be ligated together by the PreCR Reaction Mix?
  25. Can T4 DNA ligase be used to ligate across an abasic site?
  26. How are abasic sites repaired by the PreCR Reaction Mix? Are new nucleotides put in and ligated? Is the existing nucleotide repaired?

Troubleshooting

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