Product Class: Other

T7 Exonuclease
NEB4 cloned at NEB recombinant 25 No

Product Introduction

  • Double-stranded DNA specific exonuclease
  • Initiates at the 5' termini of linear or nicked double-stranded DNA
  • Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

T7 Exonuclease is ideal for:

  • Site-directed mutagenesis
  • Nick-site extension 
Catalog # Size Concentration
M0263S 1000.0 units 10000 units/ml
M0263L 5000.0 units 10000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Protocol for T7 Exonuclease (M0263)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

Research Posters

FAQs & Troubleshooting

FAQs

  1. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
  2. How Does T7 Exonuclease differ from Exonuclease III (NEB# M0206)?
  3. What is the activity of T7 Exonuclease in the NEBuffers?
  4. Can T7 Exonuclease be heat inactivated?
  5. What is the best way to generate partials using T7 Exonuclease?
  6. Can DNA be blunted using T7 Exonuclease?
  7. Will T7 Exonuclease work in rCutSmart buffer?
  8. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

Tech Tips

Addition of 6 phosphorothioates to the 5’ end of DNA can block activity up to 90%.

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