Product Class: Other

EcoRI Methyltransferase
neb31 cloned at NEB recombinant 37 65 Heat SAM

EcoRI Methyltransferase is now supplied with rCutSmart Buffer (12/1/2021) 

M0211EcoRIMethyl_thumb

Product Introduction

  • EcoRI Methyltransferase modifies the internal adenine residue (N6) of the sequence GAATTC
  • Supplied with rCutSmart Buffer and 32 mM S-adenosylmethionine (SAM)
Catalog # Size Concentration
M0211S 10000.0 units 40000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Recommended Protocol for 3H labeling of DNA
  2. Recommended Protocol for Methylation of Genomic DNA

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
  2. What should be considered if the methylation is not going to completion?
  3. Can EcoRI Methyltransferase be heat inactivated?
  4. Does EcoRI Methyltransferase require MgCl2?
  5. Will EcoRI methylation block the ApoI restriction enzyme?
  6. Will any NEB methyltransferases (methylases) work on RNA?