Product Class: Kit

Phusion® High-Fidelity PCR Kit

Product Introduction

Ideal for high speed and high-performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

  • 50X higher fidelity than Taq
  • Robust Reactions - maximal success with minimal optimization
  • Kit includes everything needed for a PCR reaction, including controls
Catalog # Size Concentration
E0553S 50.0 reactions
E0553L 200.0 reactions

Protocols, Manuals & Usage

Protocols

  1. PCR Optimization of the Control Template using Phusion® High-Fidelity PCR Kit
  2. Protocol for a Routine PCR with Phusion® High-Fidelity PCR Kit
  3. PCR Optimization with Phusion® High-Fidelity PCR Kit

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What are the differences between the various available Phusion® products?
  2. My results are not as expected. Where can I find troubleshooting help?
  3. What ends will my PCR products have?
  4. What does exonuclease activity mean for a DNA polymerase?
  5. Does Phusion® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
  6. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  7. How should I determine the appropriate annealing temperature for my reaction?
  8. What should the final primer concentration be in my PCR?
  9. My template is GC-rich or supercoiled. How can I optimize my product yield using Phusion® High-Fidelity DNA Polymerase?

Troubleshooting

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