Principle
Improved RNA quality from formalin-fixed, paraffin-embedded samples
NucleoSpin® FFPE RNA is designed for purifying RNA from formalin-fixed, paraffin-embedded (FFPE) samples. In the first stage, paraffin is removed from the sample, utilizing the odor-free Paraffin Dissolver*. Then, the sample is lysed releasing the nucleic acids. Subsequent incubation of the RNA with D-Link Buffer effectively removes crosslinking from RNA and reverses formaldehyde modifications. The decrosslinking step takes only 15 minutes and RNA can be purified by binding to the NucleoSpin® FFPE column. NucleoSpin® FFPE RNA kits allow elution of RNA in as little as 5 µL, resulting in higher concentration compared to other manufacturer’s kits.
Isolated RNA is high in concentration, formaldehyde crosslinks are removed efficiently, and RNA integrity is well preserved during the complete process (deparaffinization, decrosslining, purification). Thus, RNA shows good performance in typical downstream applications like RT-PCR.
XS-column design – increased sensitivity
NucleoSpin® FFPE RNA columns are designed for increased sensitivity. A silica membrane of small diameter is embedded in a funnel-shaped ring (see column cross-section), allowing an elution volume of only 5–30 μL.
* Users preferring xylene deparaffinization will find protocols utilizing xylene for deparaffinization in the manual. Deparaffinization using xylene shows the same efficiency as deparaffinization using Paraffin Dissolver. Xylene is not included in the kit.
Features
| Technology |
Silica-membrane technology |
| Format |
Mini spin columns-XS design |
| Sample material |
≤ 7 sections (10 μm) of 250 mm2 total area (< 15 mg paraffin**) |
| RNA yield |
Depending on amount and quality of the sample |
| Purity (A260/280) |
1.9-2.1 |
| Typical RIN (RNA integrity number) |
Strongly depending on sample quality (2–6) |
| Elution volume |
5 - 30 µl |
| Preparation time |
70 min/6 preps |
| Binding capacity |
110 µg |
** When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with Xylene for deparaffinization.
