The Fastest Real-Time PCR Combined with the most Accurate Hi-Res Melting®
The LightScanner 32 (LS32™) instrument is an air thermocycler capable of automatically analyzing samples for the presence of targeted nucleic acid. It combines real-time, rapid thermocycling technology and high resolution melting (HRM) and is capable of 20 minute PCR protocols. The LS32 instrument is configured for three-color detection for real-time and single-color detection for high resolution melting (HRM). Real-Time PCR results can be generated using a variety of labeled probe chemistry including SYBR® Green, hydrolysis probes, HybProbes®, and others. Optimal high resolution melting (HRM) results are generated using LCGreen® Plus dye.
A computer is included with the system that comes pre-loaded with easy-to-use run and analysis software. The software automatically collects and interprets data, then reports the results.
Temperature Control
Ambient air is pulled into the instrument by the intake fan, causing positive pressure to force the air through the heating duct and into the carousel chamber, where it is dispersed in the chamber by the secondary chamber fan. Cooling is done in a similar manner by forcing unheated ambient air into the chamber to cool the samples. This method allows the fastest temperature transitions in the world, allowing PCR to be routinely completed in 15-20 minutes.
Real-Time Fluorimeter
A three-channel fluorimeter block is used for the real-time detection of the dyes/probes in each amplification reaction. The fluorimeter reads and stores data for each sample simultaneously in all three channels with every fluorescence acquisition. Excitation of each sample is from a light emitting diode (LED) that is filter tuned and passed through a dichroic mirror at 470 nm. The three detection channels are filter tuned and correspond to 530 nm, 640 nm, and 710 nm, respectively.
For fluorescence excitation, the instrument is equipped with an LED light source. This LED requires no maintenance and has an extremely long life span. The fluorimeter provides three channels, which detect emitted light with varying wavelengths. Because of the high quality optics, the measurement is both extremely fast and sensitive. In a normal amplification run, measurement of a single sample takes about 20 milliseconds.
High Resolution Melting (HRM)
Hi-Res Melting (HRM) analysis with the dye LCGreen Plus identifies heterozygous single-base changes in PCR products with a sensitivity and specificity superior to non-homogeneous techniques. Variants are detected independent of their position within the fragment. Sensitivity is highest in fragments 400 bp or fewer. In addition to identifying anonymous heterozygous variants, Hi-Res Melting can often identify the specific mutation, in such cases scanning and genotyping can be combined into one simple melting analysis.
In the LS32, an HRM is performed after PCR. A temperature controlled melter optically positions itself under the sample to be melted then ascends (via a protective guide) onto the capillary and thermally denatures (melts) the PCR product while simultaneously collecting high-density fluorescence data. When the target denaturation temperature has been reached, the melter lowers itself off the sample while being cooled by a high-velocity fan. The process repeats until all samples have been melted. The ingot then lowers, which initiates the cooling process, and moves to the next capillary. The LS32 is unsurpassed in its temperature control and data acquisition rates. With superb sensitivity and specificity, this instrument is designed to meet the needs of any scanning or genotyping project.
Specifications
Sample Description
Sample capacity: 10 µL samples
32 sample carousel
Fluorescence Acquisition for Real Time PCR
Blue LED excitation with peak emission of 470 nm
3 channel detection at 530 nm, 640 nm, and 705 nm
Fluorescence Acquisition for Hi-Res Melting (Highly optimized for LCGreen Plus)
Blue LED excitation with peak emission of 450 nm
Single channel detection at 500 nm
Performance Parameters
Temperature control range: 40 ºC – 99 ºC
Analysis time: ≤ 30 minutes
Size: (w x d x h) 12” (30.4 cm) x 16” (40.6 cm) (D) x 16” (40.6 cm)
Weight: 11.3 kg/25 lbs
Applications
Real-time PCR: Absolute quantification
Melting: Labeled probe genotyping
Hi-Res Melting: Mutation scanning
Hi-Res Melting: Unlabeled probe genotyping
Hi-Res Melting: Small amplicon genotyping
Power Requirements
110/220 V, 50/60 Hz, 650 W (auto switching power supply)
Certifications: CE (for Electrical Safety)
To obtain more information about the LightScanner 32 instrument and its capabilities, or to request a demonstration for your organization, please contact us!
