X Direct PCR Direct PCR

Finnzymes' Direct PCR – forget DNA purification

Finnzymes’ novel approach, Direct PCR, saves you time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Finnzymes' Phusion High-Fidelity DNA Polymerases and Phire Hot Start II DNA Polymerase. These unique fusion enzymes have been engineered to incorporate a double-stranded DNA binding domain that gives them extraordinary properties. Phusion and Phire DNA Polymerases are exceptionally tolerant of many PCR inhibitors present in various sample material. This allows robust amplification in conditions where conventional DNA polymerases, like Taq or Pfu, are completely inhibited. 

PCR directly from various human sample tissues

NEW! Phusion Human Specimen Direct PCR Kit contains a complete set of optimized reagents for varoius sample types, such as buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth and skin biopsies. See the product page for examples and additional information

PCR directly from animal tissues

Phire Animal Tissue Direct PCR Kit is a complete kit for for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. List of animal tissues tested with Phire Animal Tissue Direct PCR Kit (updated November 2009) Recommended sample sizes for different sample types Control primer sequences for zebrafish and Drosophila

 PCR directly from plants

Phire Plant Direct PCR Kit includes a complete set of optimized reagents, sampling tools and control primers for convenient amplification of plant DNA. List of plant materials tested with Phire Plant Direct PCR Kit (updated November 2009)

PCR directly from blood

Phusion Blood Direct PCR Kit is designed for amplification of DNA directly from whole blood. See the product page for examples and additional information Application note: Direct PCR from blood preserved on Whatman FTA and 903 Cards Application protocol: PCR-RFLP directly from blood using Phusion Blood Direct PCR Kit

PCR directly from formalin-fixed paraffin-embedded (FFPE) tissues

Application note: PCR from FFPE tissues without DNA extraction using Phusion DNA Polymerase Gel images - Direct PCR from prostate tissue section - Comparing performance of Phusion DNA Polymerase to Taq DNA polymerase - Comparing the simple disruption protocol to a commercial DNA extraction kit

Note that PCR from unpurified starting material may require more optimization than standard PCR reactions from purified DNA. Also, the Direct PCR approach may not be suitable for all applications. So, please read the protocols carefully when planning your Direct PCR experiments. If you have questions or comments, please don't hesitate to contact us.